Module 15 Flashcards
What are the three main approaches for 3D bioprinting?
See Slide 4
Biomimicry: This can be achieved by reproducing specific cellular functional
components of tissues, for example, mimicking the branching patterns of the
vascular tree.
Autonomous self-assembly: It requires an intimate knowledge of the
developmental mechanisms of embryonic tissue genesis and organogenesis
as well as the ability to manipulate the environment to drive embryonic
mechanisms in bioprinted tissues.
Mini-tissues: Organs and tissues comprise smaller, functional building
blocks. Mini-tissues can be fabricated and assembled into the larger construct
by rational design, self-assembly, or a combination of both.
3D bioprinting approaches
What is the typical process for bioprinting?
See Slide 5
1. Imaging (X-ray, CT, or MRI)
2. Design Approach (Biomimicry, Autonomous self-assembly, or Mini-tissues)
3. Material Selection (Synthetic Polymers, natural polymers, or ECM)
4. Cell Selection (Differentiated cells, pluripotent stem cells, or multipotent stem cells)
5. Bioprinting (inkjet, microextrusion, or Laser-assisted)
5. Application (Maturation, Implantation, and In vitro testing)
What are the advantages and disadvantages of extrusion bioprinting?
See slide 7
Advantages
1. Simple process
2. Print large scale construct
3. Selection of various materials
4. Good reproducibility
Disadvantages
1. Low speed
2. Low printing resolution
3. Low cell viability
4. Limited in bioink viscosity
What are the different types of Extrusion-based Bioprinting systems?
See slide 9
1. Pneumatic micro-extrusion
2. Mechanical micro-extrusion
3. solenoid micro-extrusion
What are the ideal material properties for bioprinting?
See slide 11
1. Printability
2. Biocompatibility
3. Degradation kinetics and byproducts;
–Degradation rates should be matched to the ability of the cells to produce their own ECM; degradation byproducts should be non-toxic.
4. Structural and mechanical properties
5. Material biomimicry
What are the three types of bioink?
See slide 12
1. Hydrogel bioinks (natural and synthetic)
2. Cell aggregate/pellet-based bioinks
3. A combination of the two as hybrid materials
What is the process of 3D-bioprinting of cell-encapsulated constructs by using silk-gelatin as a bioink?
See Figure 4 on slide 16
1. Degumming and solubilization of silk in silk fibroin solution
2. Addition of gelatin to make silk/gelatin solution.
3. Incorporate tyrosinase or sonication
4. Add cells
5. Fabrication of 3D cell-laden structure
6. In Situ crosslinking by Tyrosinase or Sonication
