Module 1.2: DNA Recombinant Technology Flashcards
Recombinant DNA are DNA molecules formed by laboratory methods of ____ (i.e., molecular cloning) in order to piece together genetic material from ____ sources
genetic recombination; multiple
True or False. Recombinant DNA can be found naturally in the genome of organisms in nature.
False.
Recombinant DNA technology comprises altering genetic material ____ an organism to obtain _____ characteristics in living organisms or as their products.
outside; enhanced and desired
Recombinant DNA technology involves the insertion of ____ from a variety of sources, having a desirable gene sequence via the appropriate_____.
DNA fragments; vector
Match the proponent/s for the discovery in the history of rRNA technology.
- Was awarded the 1986 Nobel Prize for medicine in his discoveries on growth factors.
- Developed the first recombinant DNA utilizing bacterial DNA and plasmids.
- Conceived and approach to create rDNAs in vitro
- Created the first chimeric DNA in vitro
- Isolated the new cloning vector, pSC101 and created bacterial intra- and interspecies rDNAs
Choices:
Berg and colleagues
Berg and Loban
Herbert Boyer
Stanley Cohen
Jackson and colleagues
- Stanley Cohen
- Herbert Boyer and Stanley Cohen
- Berg and Loban
- Jackson and colleagues
- Stanley Cohen and colleagues
What was the first plasmid cloning vector?
lambda DVGal 120 (an operon encoding galactose metabolism genes concatenated with simian vacoulating virus 40)
This hormone discovered by Stanley Cohen is involved in the proliferation and differentiation of cells in vivo.
Nerve growth factors and epidermal growth factors
Memorize/familiarize
- An approach to creating rDNA in vitro is conceived
- The first bacterial cloning vector was isolated
- First chimeric DNA is created
- Second cloning vector is isolated
- First intentional creation of rDNA molecules
- Ribosomal genes propagated in E. coli cells
- Asilomar conference looked at safety and containment issues of extrachromosomal DNAs and its risks of cancer
- First gene transfer with humans
- Gene therapy products are regulated by FDA
- First approved gene transfer study on a chile with SCID
- Gelsinger’s death
- FDA elevates administrative unit that evaluates cellular, tissue, and gene therapy products.
Genetic engineering refers to the process of using ____ to modify an organism’s ____ to achieve ____.
recombinant DNA technology; DNA; desirable traits
Addition of ____ in the form of ____ that are generated by ____ is the most common method of genetic engineering
foreign DNA; recombinant DNA vectors; molecular cloning
Other terms that are synonymous with genetic engineering
Recombinant DNA technology
Gene manipulation
Gene modification
____ is the production of an exact copy—specifically, an exact genetic copy—of a gene, cell, or organism
DNA Cloning
____ allows for the creation of multiple copies of genes, expression of genes, and study of specific genes
Molecular cloning
_____ is a method used to make a clone or an identical copy of an entire multicellular organism.
Reproductive cloning
____ is a combination of DNA fragments generated by ____ that does not exist in nature.
Recombinant DNA; molecular cloning
Two types of enzymes in creating recombinant DNA.
restriction endonuclease; ligase