Module 1: Lesson 1 Flashcards
1
Q
What does BHAG stand for?
A
Big Hairy Audacious Goal
2
Q
What is the “top down” strategy?
A
To sequence the minimum amount of genes, generating a very small amount of high quality sequence with minimum overlap.
3
Q
What is the first step of sequencing?
A
To creating a map. This started with making a chromosome map, then a genetic map, then a physical map.
4
Q
What are three ways to amplify a signal?
A
radioactivity, cloning, PCR
5
Q
What are challenges to clonal amplification?
A
Unclonable regions (repeats and organism toxicity) and genomics rearrangements and alterations
6
Q
What are challenges to PCR?
A
Thermostable polymerase, accurate polymerase, and efficient cycling
7
Q
A
