Module 1 Flashcards
What is the theory of spontaneous generation?
Life arises spontaneously from nonliving material
Name some of the detractors of spontaneous generation, who went on to disprove the theory.
Francisco Redi, Louis pasteur, John Tyndall
What did Francesco Redi do for his experiment?
He was an Italian biologist. The demonstrated worms and rotting meat came from exit flies, landing on the meat.
True or false the beneficial microbes can become pathogens
True
True or false less than 1% of all microbial species can be grown in studied in the laboratory
True
What is biodegradation?
The process by which organic substances are decomposed by micro organisms into simpler substances, such as carbon dioxide, water, and ammonia
What is bioremediation?
Using micro organisms to hasten decay of pollutants
Bacteria, synthesize, commercially, valuable products list, an example
 Amino acids into dietary supplements
True or false genetic engineering includes: the introduction of jeans into another organism, disease, resistant plants, production of medication’s
True
All living things can be classified into one of the three groups or domains. What are these domains called?
Bacteria, archaea, eucarya
List characteristics of bacteria
Single, celled, prokaryotes, no membrane, bound nucleus, no other membrane, bound organelles, DNA in nucleoid, most have a specific shape, multiply via binary fission, move with flagella
Where is DNA kept in bacteria?
The nucleoid
What is archaea classified as?
Prokaryotes
How do archaea multiply?
Binary fission
Describe the characteristics of eukaryotes
They have a true nucleus, membrane bound, organelles there more complex than prokaryotes microbial members include fungi, algae, and protozoa
True or false algae have Richard cell walls
True
True or false algae have many flagella
True
True or false fungi can be single celled or multicellular
 True
True or false protozoa are prokaryotes
False, they are eukaryotes
True or false can eukaryotes be single celled
True
Are protozoa, larger or smaller than prokaryotes
Larger
True or false protozoa have a rigid cell wall
False
True or false protozoa are motile
True, most are motile
True or false viruses viroids and prions are all obligate intracellular agents
This is false, only viruses and viroids are obligate intracellular agents.
What are prions?
They are abnormal form of a cellular proteins
True or false virus consist of either DNA or RNA and are surrounded by a protein coat
True
True or false viroids consist of only RNA no protein coat
True
True or false prions consist of proteins no DNA or RNA
True
 in viruses, infected, living cells are termed blank
Hosts
How do viruses multiply
They multiply using host machinery, nutrients
True or false viruses are active outside of hosts
This is false, they are inactive, they are obligate intracellular parasites
True or false viroids are simpler than viruses
True
True or false viroids require host cells for replication
True
True or false viroids can cause plant diseases
True
True, or false viroids, or simpler than viruses
True
What are prions
Infectious proteins, Miss folded versions of normal cellular proteins, found in brain
In prions, abnormal proteins, bind to form blank
Fibrils
How do you prions function?
Prions, cause miss folded version to force the normal version of miss fold in the cells are unable to function of normal proteins. Bind to form form fibrils.
Name the three infectious agents
Viruses, viroids, prions
What are protists?
A eukaryotic organism that is not an animal plant or fungus. An example is algae or protozoa.
Are protists multicellular or unicellular
They are unicellular eukaryotes
Objective lens forms, blank image
Real
In magnification, ocular lens forms the blank image
Virtual
True, or false shorter wavelengths provide a better resolution
True
What are dark field microscopes?
They are microscope’s that directs light towards specimens at an angle, only like scattered by specimen, enters, objective lens, cells appear as bright object against the dark background
Blank microscope allows visible light to pass through a series of lenses to produce a magnified image. They are relatively easy to use and considerably less expensive than other types of microscopes.
Light
Right field, dark field and face contractor, all types of blank microscope’s
Light
What is the most common type of microscope?
Bright Field
What is the brightfield microscope do?
Illuminates the field of view evenly
What does the dark field microscope do?
Light is directed towards the specimen, add an angle it makes unstained sells easier to see organism stand out as bright objects against the dark background
What is phase contrast light microscope?
Increases contrast by amplifying differences in refractive index. It makes unsustained cells easier to see.
What is the differential interference contrast microscope?
It’s a type of light microscope that has two beams of light, passing through the specimen, and then it re-combines. The image of the specimen appears three-dimensional.
What type of microscope shows the specimen as three dimensional
Differential interference contrast
What type of microscope projects ultraviolet light causing fluorescent molecules in the specimen to omit longer wavelengths light. It is used to observe cells stained, or tagged with a fluorescent dye.
Florescence microscope
What kind of microscope mirrors scan a laser beam across successive regions and planes of a specimen. From the information of computer construction image
Scanning laser microscope
What are the two types of electron microscopes?
Transmission and scanning
What does a transmission electron microscope do?
Transmits a beam of electrons through a specimen
What does a scanning electron microscope do?
A beam of electrons scans back-and-forth over the surface of a specimen
What is a transmission electron microscope used?
It is used in elaborate specimen preparation is required
When is the scanning electron microscope used?
It’s used for observing surface details and produces a three-dimensional effect
What is an atomic force microscope?
Are you done? A probe moves in response to even the slightest force between it in the sample
What is the purpose of heat fixation?
He fixation adheres to the cells to the slide, and coagulates the bacterial proteins effectively killing the bacteria
True or false basic dyes are positively charged
True, the dye sticks to the specimen attracted to negatively charged cellular components.
True or false acidic dyes are negatively charged
True
What are some examples of basic dyes?
Crystal violet, Safranin
Give an example of an acidic dye?
Congo red
True or false: The dye does not stick to the specimen, it’s sticks to the background
This is true
True or false heat fixation is required with acidic dyes.
False
What is the difference between a simple stain and a differential stain?
Simple stains only require one single dye, differential stains, use to differently, color, dyes, the primary die in the counterstain.
What color is negative 
Bacteria?
Red or pink
What color is gram-positive bacteria?
Purple
What type of staining and is used to detect mycobacterium?
Acid fast staining
What are special stains?
They are used to emphasize certain cell parts that are not revealed by conventional staining methods
List two examples of special stains
Capsule staining and flagellar staining
What is the purpose of the capsule stain?
To reveal the presence of bacterial capsule
What is an endospore stain?
Is staying that uses heat to facility uptake of primary dye
True or false negative staining is used with basic dyes.
This is false, acidic dyes are used typically India ink, or nigrosin
Why are cells stained and light microscopy
To enhance visualization of the cell or certain cellular components under a microscope
Which of the three domains have prokaryotic type cell structures?
Bacteria
Which types microscopy allows visualization of three-dimensional cell structure?
Fluorescence
Why are cationic dyes used for general microscopy of cells?
 The cationic dyes find the bacterial cells which can be easily observed against bright background. It stains the bacteria
True or false Archaea are considered prokaryotic cells
True
What are the two most common types of prokaryotic cells specifically shape
Coccus - spherical
Rod - cylindrical
How do most prokaryotic cells divide
Binary fission
Define Diplococcus
A chain of 2 cocci
What type of shape does Neisseria gonorrhoeae have?
Diplococcus
What type of shape does Streptococcus have?
Long chains of cocci
What type of shape does Sarcina have?
Cubical packets
What type of shape does staphylococcus have?
Grape like clusters
What is myxobacteria?
A group of bacteria that predominately live in the soil and feed on insoluble organic substances 
What is glycolcalyx and where is it found in the prokaryotic cell?
Glycocalyx is a coding or layer of molecules external to the cell wall. It serves protective adhesive in receptor functions. It may fit tightly or be very loose and diffuse
Describe the properties of the bacterial chromosome or nucleoid.
The nucleoid is composed of condensed DNA molecules. DNA directs all genetic in heredity of the cell and codes for all proteins.
What is the pilus?
An elongate, hollow appendage used in transfers of DNA to other cells.
What is the plasmid on the prokaryotic cell?
It’s double stranded DNA circle containing extra jeans
True or false prokaryotic cells have a cell wall
True they have a semi rigid wall casing that provide structural support and shape for the cell
What is the actin cytoskeleton?
Long fibers of proteins that encircles the cell just inside the cell membrane and contribute to the shape of the cell
What is an endospore?
Dormant body formed within some of the bacteria that allows for their survival in adverse conditions 
What is the fimbriae?
Find hair like bristles, extending from the cell surface, that health and adhesion to other cells and surfaces
What is simple diffusion?
Movement from high to low concentration the speed depends on the concentration
What is osmosis? 
Diffusion of water across selective permeable membrane due to unequal solute concentrations 
Blank diffusion is a form of passive transport 
Facilitated
Does facilitated diffusion require energy?
No energy required
Does active transport require energy?
Yes, uses ATP and proton motive force for the two main mechanism