MODULE 1 Flashcards

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1
Q

What common features do prokaryotes and eukaryotes share?

A

They both have DNA (pro=circular, euk=linear), ribosomes, cytoplasm and a cell membrane

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2
Q

What is a prokaryote?

A

Prokaryotes aren’t membrane bound and don’t have any membrane-bound organelles. Prokaryotes have a cell membrane, a cytoplasm, ribosomes and genetic material. It doesn’t have a nucleus and have one circular chromosome. They are usually apart of unicellular organisms, either bacteria or archaea.

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3
Q

What is a eukaryote?

A

Eukaryotic cells are much more complex than prokaryotes, and are also larger in size. They have membrane bound organelles, multiple linear chromosomes and a nucleus.
Organelles are structured and carry out processes such as respiration and photosynthesis.

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4
Q

What are light microscopes?

A
  • Limited in magnification
  • Resolution of around 0.2 micrometres or 200 nm
  • Observed living cells
  • They pass light through a specimen
  • Lenses magnify the specimen
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5
Q

What is a TEM?

A
  • 2 dimensional image
  • No living specimens can be observed
  • Passes electrons through a specimen, witha strong magnetic field that acts as a lens to produce a very high definition cross section.
  • High quality, high resoltion
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6
Q

What is an SEM?

A
  • Has a large depth of field so it can be used to examine the surface structure of specimens
  • It bombards solid specimens with a beam of electrons with causes secondary electrons to be emitted from the specimen.
  • Electrons bounce off surfaces
  • It is 3 Dimensional
  • No living specimens can be observed.
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7
Q

What is the ER? How does structure relate to function?

A

-Networks of flattened, interconnected membranes, channels hat run through the cytoplasm, If something has to move throughout the cell, it is done through the ER

-Provides a connection of pathways between the nucleus and the cell’s environment, allowing for intracellular transport.
The main function of ER is transport, and also plays a role in processing cell products.

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8
Q

What is the difference between the smooth ER and the rough ER?

A

The rough ER processes proteins made by the cell and can synthesise lipids
Smooth ER is the main site of lipid production

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9
Q

What is the nucleus? How does structure relate to function?

A

Large organelle bound by a double membrane
-This membrane has tiny pores that regulate the passage of substances between the nucleus and the cytoplasm.

Controls the activity of the cell
-The tiny pores allow communication from the nucleus and the cytoplasm.

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10
Q

What is the mitochondrion? How does structure relate to function?

A

-The inner membrane (cristae) is heavily folded and has an increased surface area.

The increased surface area allows for the attachment of enzymes responsible for energy production within the cell. Therefore, more surface area equals more energy production.

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11
Q

What are lysosomes? How does structure relate to function?

A

-Lysosomes contain digestive enzymes that are responsible for splitting compound chemical compounds into simpler ones.

The simple compounds created by the digestive enzymes are used as building blocks for new compounds and organelles (recycling)

-The membrane ruptures, releasing the enzymes inside which then digest the cell, killing it in the process.

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12
Q

What are chloroplasts? How does structure relate to function?

A

-Liquid part of the chloroplast named the stroma
-Layered membranes
Contains membrane stacks called thylakoids. Chlorophyll is found in thylakoids.
-Layering of thylakoids increases the surface area over which chlorophyll occurs, meaning more sunlight can be absorbed for photosynthesis.

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13
Q

What is the golgi body? How does structure relate to function?

A

-Flat membranes, flattened sacs that bud off into vesicles

The vesicles are the secretory function of the golgi

-Modifies proteins and packages them for cell export, and also determining where they will go, it can be transported into the cell or out.

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14
Q

What is a ribosome?

A

Ribosomes are the site of protein synthesis within the cell. Ribosomes are made of RNA and protein, and they are the machinery that carries out genetic instructions of DNA to produce any proteins that are needed for cell functioning and structure.

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15
Q

What is the fluid mosaic model?

A

The membrane is composed of a bi layer of phospholipids in which proteins and other molecules are suspended. The materials needed by the cell or any waste are able to cross the membrane via trans membrane proteins.

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16
Q

What is a selectively permeable membrane?

A

Allows certain molecules to pass through the microscopic pores. For example, it will let water (solvent) out but may stop dissolved particles (solute) from passing through.

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17
Q

15.What are the structures in the cell membrane that are responsible for flexibility, the movement of ions into cells and the ability to bind hormones.

A
  • Lipid component- flexibility and self repair
  • Membrane proteins suspended in the bi-layer- act as pores or form active carrier systems for transport- allow the exchange of substances between the cell and the cell environment. Ions use these channels to engage in facilitation diffusion into the cells.

receptor proteins- ause the cells to respond to certain stimuli and signals.

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18
Q

Why is it called the fluid mosaic model?

A

The fluid component of the model is the phospholipid bilayer. It is not rigid in structure, but very fluid. It has the ability to flow and change shape. The phospholipid bilayer is referred to as a mosaic as there are various molecules and proteins that aer embedded into the lipid bilayer, like a mosaic. The floating proteins are the mosaic.

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19
Q

What is diffusion? What is facilitated diffusion?

A

Simple diffusion- passive net movement of a substance from a region with high concentration to a region of low concentration until equilibrium is reached- passively pass through the cell membrane. -Facilitated diffusion-large molecules and charged particles do not readily pass through the phospholipid bilayer and require carrier and channel proteins to assist them in diffusing into the cell.

20
Q

What is osmosis?

A

It is the net movement of solvent molecules from a region of high solvent concentration to low solvent concentration through a semi-permeable membrane. Osmosis requires no energy input.

21
Q

What is active transport?

A

active transport is when a molecule needs to be moved from an area of low concentraion, to an area of high concentration, going against the concentration gradient. It has to be moved across the membrane through ATP energy. It requires input energy, unlike osmosis.

22
Q

What are the two forms of bulk transport?

A

endocytosis is the process in which large molecules or particles are exported by a cell by the fusion of a vesicle within the cell membrane. Contents are released to the exterior of the cell.

Exocytosis is the reverse of endocytosis. The cell membrane furrows or engulfs around a particle, making it enter the cell. This requires energy expenditure and can be categorised into pinocytosis, and phagocytosis.

23
Q

Why is carbon such an important element to life?

A

Carbon is the functional element for living things because it’s able to bond in many different ways. It can form many compounds that are essential to life.

24
Q

What are the inorganic substances that are important to cells?

A

Water- it is the transport medium in cells
Mineral salts- assist all chemical reactions, used in the synthesis of macromolecules and body tissues
Gases- carbon dioxide: used during photosynthesis, released as a product of aerobic cellular respiration.
Oxygen: used by all living organisms during aerobic cellular respiration, photosynthesis product

25
Q

What is an enzyme? What is a substrate?

A

An enzyme is a protein that acts as a catalyst, speeding up a chemical reaction whilst remaining unchanged.

A substrate is the substance which an enzyme acts on/

When the substrate has acted on and changed the substrate, the substances that result are called the product.

26
Q

How do enzymes work?

A

Enzymes speed up chemical reactions by lowering the activation energy required for the reaction to start.

27
Q

What is the lock and key model?

A

Believed the active site was rigid and the substrate model is reciprocally shaped.

28
Q

What is the induced fit model?

A

Proteins aren’t rigis. The binding of the substrate to the active site of the enzyme induces the enzyme to alter its shape to better fit the substrate.

29
Q

How does temperature effect enzyme activity?

A
  • Each enzyme has an optimal temperature, below this temperature, the rate of reaction will be slowed.
  • If the temperature is a few degrees above the optimal temperature, the enzyme will become denatured.
  • Excessive cold can also cause denaturation
30
Q

How does pH effect enzyme activity?

A
  • Enzymes are pH sensitive
  • Each enzyme has an optimum pH, the pH that it functions at most efficiently and effectively
  • If the pH is higher or lower than the optimal pH, the rate of enzyme reactions are slowed.
  • Extremes of pH can cause the enzyme to denature
  • Usually functions near neutral (except for digestion)
31
Q

How does substrate concentration effect enzyme activity? How does enzyme concentration effect enzyme activity?

A

As the concentration of the substrate increases, so does the rate of the enzyme catalysed reaction. T this point adding more substrate will not increase the rate of reaction (saturation point)

More enzynes=more reactions

32
Q

What is the efficiency of exchange? How does it relate to SA:V?

A

HIGH SA VOLUME= diffusion meets the needs of the organisms (more efficient exchange of materials in their environment, less work)

LOW SA V= diffusion unable to meet the need of the organism- specialisation required.

33
Q

What is aerobic cellular respiration?

A

A series of chemical cellular reactions that convert biochemical energy from glucose to chemical energy in the form of ATP.

It occurs in the mitochondria (Matrix and Cristae)

34
Q

What is anerobic respiration? (Lactic Acid Fermentation)

A

No oxygen is needed. Occurs through either alcohol fermentation. on or lactic acid fermentation.

Alcohol fermentation- breakdown of glucose in the absence of oxygen to form ethanol, carbon dioxide and 2ATP.

Lactic acid fermentation-carried out when an organism cant produce enough energy by aerobic cellular respiration. (strenuous exercise- glucose is broken down in the absence of oxygen)

35
Q

What is the difference in the efficiency of aerobic respiration and fermentation?

A

Aerobic cellular respiration is much more efficient, but fermentation is much faster. Anaerobic respiration is a waste of energy, but its really fast.

36
Q

What is cell waste?

A

The products of the reactions in the cell that aren’t required by the organisms. These can be eliminated or used in other cells etc.

Carbon dioxide is a byproduct of cellular respiration, and is eliminated by the respiratory system.

37
Q

What is a co-factor and how do they affect enzyme activity?

A

A cofactor is a substance other than the subrate in the active site that is necessary for the activity of an enzyme. They increase the efficiency of function, helps them bind to the active site.

38
Q

What is an inhibitor and how do they affect enzyme activity?

A

A substance that binds to an enzyme and decreases its activity

39
Q

What is an inhibitor and how do they affect enzyme activity?

A

They bind to an enzyme and decrease its activity.

40
Q

What does isotonic mean?

A

Fluids inside and outside of a cell are of equal solute concentration- pressure is created

41
Q

What does hypertonic mean?

A

A solution of higher solute concentration (lower water concentration) that surrounds a cell, movement of water molecules is out of the cell

42
Q

What does hypotonic mean?

A

A solution of lower concentration (high water concentration) that surrounds a cell- net movement of molecules will be into the cell.

43
Q

Blood cells were placed in three different solutions. After some time, cells in solution X became shrivelled. Cells in solution Y burst and cells in solution Z remained the same. What were the solutions?

A

Solution X- Hypertonic
Solution Y- Hypotonic
Solution Z-Isotonic

44
Q

What are the biomacromolecules?

A

n

45
Q

How can energy enter the cell?

A

Through light energy or chemical energy as a result of digested food.

46
Q

Aerobic cellular respiration equations

A

C6H12O6 + 6O2 → 6CO2 + 6H2O + 38 ATP

glucose + oxygen → carbon dioxide + water + usable energy

47
Q

Photosynthesis equations

A

6CO2 + 6H2O → C6H12O6 + 6O2.

carbon dioxide + water —> glucose + oxygen + water