Modelorganisms Flashcards
What are model organisms
.organisms that we can use in the lab as representative systems
Name 5 model organisms for cell biology
Nematode, fruit fly, zebrafish, house mouse, human
What is a nematode
It’s a multicellular invertebrate that lives in soil
Why are nematodes good model organisms
Transparent body for imaging, inexpensive, short lifespan, can be frozen, nervous system can be easily seen in fluore fluorescence microscopy
Why are fruit flies good model organisms and what is its genome size
Small, easy, fast, genome =13,000
What ave zebrafish, why ave they good model organisms, and what’s its gene size
Smau, transparent fish, has vertebrate, 26,000 genes
Why ave mice model organisms and what’s its gene size
Genetic and disease similarity to humans, 22,000 genes
What’s a primary culture
CW cells obtained directly from tissue or organ
What’s a secondary culture, how is it stored
A culture of cells previously established. Stored in liquid nitrogen
What was the HeLa cell
First human cen grown in a culture, used as a vaccine for polio
What ave nutrient requirements of cultured animal cells
Energy source, aa mixture, pH buffer and indicator, antibiotics (to stop bacterial growth)
Cultured animal cells are classified as what? (2 names)
Adherent or suspension
Pros and cons of adherent cells
Hard to culture, grow in small volumes, easy to image
Pros and cons of suspension cells
Easy to culture, grow in large volumes, hard to image
What do cultured cells allow us to do
Preform cell-based assays without the need for the entireorganism
