Fluorescence Flashcards
1
Q
Why do we need to use fluorescence?
A
To study individual organelles that are living
2
Q
Now to makefluorescencein cells
A
Add chemicals that have fluorescentproperties
3
Q
Immunofluorescence
A
Antibodies used to detect proteins
4
Q
Limitations of immunofuorescence
A
-You have to raise an antibody against your protein
- hard to add antibodies to living cells
5
Q
What is GFP and where is it from
A
From jellyfish, its A fluorescent protein
