Model organisms 2

Question 1

How is DNA inserted into Arabidopsis

Answer 1

TDNA can be inserted by the bacterium Agrobacterium tumeficians

Question 2

In what manner is TDNA inserted into the arabidopsis genome?

Answer 2

It is inserted randomly into the genome via non homologous recombination

Question 3

What gene was targeted in the diabetic mouse experiment?

Answer 3

PDX1 homeotic gene for pancreatic cells and insulin production transcriptionally activated in liver cells

Question 4

What makes up the MPH transcriptional module in the diabetic mice experiments

Answer 4

A transgenic mouse already encoding Cas9, dgRNAs (shorter than normal gRNAs so Cas does not cut) and MPH transcriptional activator

Question 5

What functional problem creates the disease phenotype SCA1

Answer 5

CAG expanded repeats in the SCA-1 gene - causing polyglutamine tract expansion in the ataxin protein

Question 6

What two factors are key in designing a target vector to be implanted in mice blastocyst?

Answer 6

Positive and negative markers - NeoR and HIV-TK