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MBB262 > CRISPR cas > Flashcards

CRISPR cas Flashcards

1
Q

What is an essential sequence factor for a CRISPRcas system to function?

A

PAM sequence must exist endogenous to target sequence

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2
Q

What is the target locus when developing malaria resistant mosquitos?

A

KH^w (encoding kynurenin hydroxylase enzyme)

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3
Q

What does the vector plasmid contain?

A
  1. U6A promoter expressing gRNA
  2. Cas9 Nuclease flanked with known promoters of the A.stephensi genome
  3. DSRED dominant visible marker
  4. Dual antipathogen gene for resistance against plasmodium falciparum
  5. homologous DNA fragment to KHw locus
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4
Q

How can CRISPRcas be used beyond gene editing

A

Deactivating Cas9 makes it a versatile RNA guided DNA targeting tool for regulating and imaging the genome

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MBB262 (7 decks)

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