Model Organisms

Question 1

How to process reverse genetics?

Answer 1

• start with gene sequence information
  -engineer a loss of phenotype to evaluate gene to function

Question 2

What is the starting and ending point of forward genetics?

Answer 2

Starting point— mutant animal
Ending point— determine gene function

Question 3

How does forward genetics study gene function?

Answer 3

-classical approach, gene identified by studying mutant
-gene must be cloned for further functional analysis

Question 4

How does reverse genetics study gene function?

Answer 4

-start with gene sequence information

-engineer w loss of function phenotype to evaluate gene function

Question 5

What is tetrad analysis?

Answer 5

Recombination occurs in meiosis (between non-sister chromatids otherwise it wouldn’t generate any diversity)

Question 6

Tetrad analysis is used in yeast for??
(5)

Answer 6

-location of mutations
-cloning a gene
-strain construction
-mutant analysis
-whole-genome sequencing

Question 7

Characteristics of C.elegant?

Answer 7

-stains can be frozen
-can be characterised genetically
-transparent body

Question 8

How do genetics identify genes?

Answer 8

They preform a mutagenesis screen (forward genetics)

Question 9

What is the process that causes genetics to identify genes?

Answer 9

1) mutagenised the organism to increase the likelihood of finding mutants
2) identify mutants
3) map the mutation
4) determine the molecular function of the gene product
5)determine how the gene product interacts with other gene products in a pathway

Question 10

What are 2 limitations of forward genetics?

Answer 10

• genes preforming an essential function
  -genes with redundant functions