Mod 1

Question 1

Cause of false POS or NEG in Rh typing:

- Failure to add reagent

Answer 1

False neg

Question 2

Cause of false POS or NEG in Rh typing:

- Over shaking

Answer 2

False neg

Question 3

Cause of false POS or NEG in Rh typing:

- Rouleaux

Answer 3

False Pos

Question 4

Cause of false POS or NEG in Rh typing:

- Reagent deterioration

Answer 4

Fase neg

Question 5

Cause of false POS or NEG in Rh typing:

- Cold autagglutinins

Answer 5

False Pos

Question 6

Cause of false POS or NEG in Rh typing:

- using wrong antiserum

Answer 6

False pos OR false neg

Question 7

Cause of false POS or NEG in Rh typing:

- Positive DAT on cells

Answer 7

False pos

Question 8

Cause of false POS or NEG in Rh typing:

- incorrect cell suspension

Answer 8

False Neg

Question 9

Cause of false POS or NEG in Rh typing:

- Warm autoagglutinins

Answer 9

False pos

Question 10

Cause of false POS or NEG in Rh typing:

- Polyagglutinable cells

Answer 10

False Pos

Question 11

Cause of false POS or NEG in Rh typing:

- blocking of antigen sites (HDFN)

Answer 11

False neg

Question 12

Cause of false POS or NEG in Rh typing:

- Incorrect antiserum-to-cell ratio

Answer 12

False Neg

Question 13

Cause of false POS or NEG in Rh typing:

- using a post-transfusion sample of an Rh = patient who received Rh + blood

Answer 13

False Pos

Question 14

Cause of false POS or NEG in Rh typing:

- contaminated reagent `

Answer 14

False Pos OR False Neg

Question 15

””"”Cause of false POS or NEG in AGT typing:

- over centrifugation or over reading

Answer 15

False Pos

Question 16

””"”Cause of false POS or NEG in AGT typing:

- too short incubation

Answer 16

False neg

Question 17

””"”Cause of false POS or NEG in AGT typing:

- improper storage of cells, serum, reagent

Answer 17

False neg

Question 18

””"”Cause of false POS or NEG in AGT typing:

- Dirty glassware

Answer 18

False pos

Question 19

””"”Cause of false POS or NEG in AGT typing:

- Test cells have positive DAT

Answer 19

False pos

Question 20

””"”Cause of false POS or NEG in AGT typing:

- Omission of AHG

Answer 20

False neg

Question 21

””"”Cause of false POS or NEG in AGT typing:

- bacterial contamination

Answer 21

False pos

Question 22

””"”Cause of false POS or NEG in AGT typing:

- Contaminated AHG

Answer 22

False Pos

Question 23

””"”Cause of false POS or NEG in AGT typing:

- centrifugation over or under

Answer 23

False Neg

Question 24

””"”Cause of false POS or NEG in AGT typing:

- improper washing

Answer 24

False Neg

Question 25

””"”Cause of false POS or NEG in AGT typing:

- Contamination

Answer 25

False neg

Question 26

””"”Cause of false POS or NEG in AGT typing:

- Elution of antibody

Answer 26

False neg

Question 27

””"”Cause of false POS or NEG in AGT typing:

- Temperature

Answer 27

False neg

Question 28

””"”Cause of false POS or NEG in AGT typing:

- weak/heavy cell suspension

Answer 28

False neg

Question 29

O: percent and sugar

Answer 29

45%, fucose

Question 30

A: percent and sugar

Answer 30

40%, N-acetylgalactose amine

Question 31

B: percent and sugar

Answer 31

11%, D-galactose

Question 32

AB: percent and sugars

Answer 32

4%, (all three sugars present)

Question 33

Rh antigens: percent and Weiner

- D

Answer 33

85%, Rho

Question 34

Rh antigens: percent and Weiner

- C

Answer 34

70%, rh’

Question 35

Rh antigens: percent and Weiner

- E

Answer 35

30%, rh’’

Question 36

Rh antigens: percent and Weiner

- c

Answer 36

80%, hr’

Question 37

Rh antigens: percent and Weiner

- e

Answer 37

98%, hr’’

Question 38

DISCREPANCIES: extra in the forward

Answer 38

1. Rouleaux
2. Cold Auto Antibodies
3. Aquired B-like antigen
4. Polyagglutination

Question 39

DISCREPANCIES: Mixed field in the forward

Answer 39

1. Recent transfusion with ‘out-of-group’ blood
2. Bone marrow transplant
3. Exchange transfusions
4. Fetal-maternal Bleeding
5. A3 or B3 subgroups
6. Chimeras

Question 40

DISCREPANCIES: Weak/Missing in the forward

Answer 40

1. Subgroups of A or B

Question 41

DISCREPANCIES: Weak/missing in the reverse

Answer 41

1. Age of patient
2. Disease state
3. True Chimera twins

Question 42

DISCREPANCIES: Extra in the reverse

Answer 42

1. Rouleaux
2. Auto Abs
3. Allo Abs

Question 43

Resolution for Rouleaux in the forward

Answer 43

Wash cells 4-6 times

Question 44

Resolution for Cold auto abs in the forward

Answer 44

Warm saline wash 4-6 times

Question 45

Resolution for Acquired B-like Ags

Answer 45

Check patient diagnosis for GI tract disease

Question 46

Resolution for Polyagglutination in the forward

Answer 46

Use monoclonal reagents

Question 47

Resolution for mixed field reactions in the forward

Answer 47

Check patient history

Question 48

Resolution for subgroups of A or B in the forward

Answer 48

• test with A1 lectin (will be + if A1)
• test with anti-a,b (will be + if Ax or Bx)
• Secretor studies

Question 49

Resolutions of Weak/missing reactions in the reverse

Answer 49

increase serum to cell ratio

• incubat at RT
• Incubate at 4 degrees
• Use enzyme treated reverse cells

Question 50

Resolution to rouleaux in reverse

Answer 50

saline replacement technique

Question 51

Resolution to auto Abs in reverse

Answer 51

Pre-warm testing

Question 52

Resolution to Allo Abs in reverse

Answer 52

• Prove a subgroup with 3+3 rule

- antibody screen