Mod 1 Flashcards
Cause of false POS or NEG in Rh typing:
- Failure to add reagent
False neg
Cause of false POS or NEG in Rh typing:
- Over shaking
False neg
Cause of false POS or NEG in Rh typing:
- Rouleaux
False Pos
Cause of false POS or NEG in Rh typing:
- Reagent deterioration
Fase neg
Cause of false POS or NEG in Rh typing:
- Cold autagglutinins
False Pos
Cause of false POS or NEG in Rh typing:
- using wrong antiserum
False pos OR false neg
Cause of false POS or NEG in Rh typing:
- Positive DAT on cells
False pos
Cause of false POS or NEG in Rh typing:
- incorrect cell suspension
False Neg
Cause of false POS or NEG in Rh typing:
- Warm autoagglutinins
False pos
Cause of false POS or NEG in Rh typing:
- Polyagglutinable cells
False Pos
Cause of false POS or NEG in Rh typing:
- blocking of antigen sites (HDFN)
False neg
Cause of false POS or NEG in Rh typing:
- Incorrect antiserum-to-cell ratio
False Neg
Cause of false POS or NEG in Rh typing:
- using a post-transfusion sample of an Rh = patient who received Rh + blood
False Pos
Cause of false POS or NEG in Rh typing:
- contaminated reagent `
False Pos OR False Neg
””"”Cause of false POS or NEG in AGT typing:
- over centrifugation or over reading
False Pos
””"”Cause of false POS or NEG in AGT typing:
- too short incubation
False neg
””"”Cause of false POS or NEG in AGT typing:
- improper storage of cells, serum, reagent
False neg
””"”Cause of false POS or NEG in AGT typing:
- Dirty glassware
False pos
””"”Cause of false POS or NEG in AGT typing:
- Test cells have positive DAT
False pos
””"”Cause of false POS or NEG in AGT typing:
- Omission of AHG
False neg
””"”Cause of false POS or NEG in AGT typing:
- bacterial contamination
False pos
””"”Cause of false POS or NEG in AGT typing:
- Contaminated AHG
False Pos
””"”Cause of false POS or NEG in AGT typing:
- centrifugation over or under
False Neg
””"”Cause of false POS or NEG in AGT typing:
- improper washing
False Neg
””"”Cause of false POS or NEG in AGT typing:
- Contamination
False neg
””"”Cause of false POS or NEG in AGT typing:
- Elution of antibody
False neg
””"”Cause of false POS or NEG in AGT typing:
- Temperature
False neg
””"”Cause of false POS or NEG in AGT typing:
- weak/heavy cell suspension
False neg
O: percent and sugar
45%, fucose
A: percent and sugar
40%, N-acetylgalactose amine
B: percent and sugar
11%, D-galactose
AB: percent and sugars
4%, (all three sugars present)
Rh antigens: percent and Weiner
- D
85%, Rho
Rh antigens: percent and Weiner
- C
70%, rh’
Rh antigens: percent and Weiner
- E
30%, rh’’
Rh antigens: percent and Weiner
- c
80%, hr’
Rh antigens: percent and Weiner
- e
98%, hr’’
DISCREPANCIES: extra in the forward
- Rouleaux
- Cold Auto Antibodies
- Aquired B-like antigen
- Polyagglutination
DISCREPANCIES: Mixed field in the forward
- Recent transfusion with ‘out-of-group’ blood
- Bone marrow transplant
- Exchange transfusions
- Fetal-maternal Bleeding
- A3 or B3 subgroups
- Chimeras
DISCREPANCIES: Weak/Missing in the forward
- Subgroups of A or B
DISCREPANCIES: Weak/missing in the reverse
- Age of patient
- Disease state
- True Chimera twins
DISCREPANCIES: Extra in the reverse
- Rouleaux
- Auto Abs
- Allo Abs
Resolution for Rouleaux in the forward
Wash cells 4-6 times
Resolution for Cold auto abs in the forward
Warm saline wash 4-6 times
Resolution for Acquired B-like Ags
Check patient diagnosis for GI tract disease
Resolution for Polyagglutination in the forward
Use monoclonal reagents
Resolution for mixed field reactions in the forward
Check patient history
Resolution for subgroups of A or B in the forward
- test with A1 lectin (will be + if A1)
- test with anti-a,b (will be + if Ax or Bx)
- Secretor studies
Resolutions of Weak/missing reactions in the reverse
increase serum to cell ratio
- incubat at RT
- Incubate at 4 degrees
- Use enzyme treated reverse cells
Resolution to rouleaux in reverse
saline replacement technique
Resolution to auto Abs in reverse
Pre-warm testing
Resolution to Allo Abs in reverse
- Prove a subgroup with 3+3 rule
- antibody screen
