Mock corrections Flashcards
Inorganic Ions
-Calcium Pectate- cell walls
•Magnesium- enzymes and chlorophyll
•Iron- haemoglobin and electron carriers
•Hydrogen Carbonate- Buffer
•Nitrate- amino acids, nucleic acids and chlorophyll
•Phosphate- phosphate salts, buffer, cell membranes and nucleotides
Structure of an amino acid
•AMINO group- NH2
•HYDROGEN group- H
•CARBOXYL group- COOH
•VARIABLE group- R
PRIMARY Structure
The sequence of amino acids in the polypeptide chain- held tg by peptide bonds
SECONDARY Structure
The way the polypeptide chain coils/ folds- form a- helix or B pleated sheet
HYDROGEN bonds- hold the structures together
Conjugated Protein
Fibrous- main function- support for a structural role- strong- due to the presence of many cross links- ‘rope’ structure
-Insoluble and stable
•3 polypeptide chains coiled around each other- triple helix
•cross link between chains and molecules create the strength
Enzymes
•TEMPERATURE- increased kinetic energy-molecules move faster- more collisions- more ESC formed
•above optimum- enzyme becomes denatured- internal HYDROGEN bonds, which maintain the active site shape, are broken
PH
As pH increases, rate of reaction increases- higher pH- enzyme becomes denatured- bonds (IONIC), determine the proteins 3D, tertiary structure/ shape become disrupted- DENATURATION
NUCLEIC ACIDS
•consist of
-pentose sugar
-nitrogenous base
-phosphate
COMPARE AND CONTRAST DNA AND RNA
•DNA- found in nucleus, RNA- found in cytoplasm
•DNA- A + T, C+ G, RNA- A+U, C+G
•DNA- deoxyribose sugar, RNA- ribose sugar
•DNA- longer/ more based
•RNA- 3 types- mRNA, tRNA and rRNA
•DNA- double chain, RNA- single chain
SEMI CONSERVATIVE REPLICATION
•Enzyme DNA HELICASE unwinds the DNA double helix by BREAKUNG THE HYDROGEN BONDS
•Each strand acts as a template for the synthesis of a new strand- exposed bases bind to free floating nucleotides in the nucleoplasm
•DNA POLYMERASE- binds the complementary nucleotides to the exposed bases on the template strand- this continues along the whole DNA- form new complementary strands
PRACTICALS
Same. Control
Alter. Independent
Measure. Dependant
Describe the mechanism of enzymes
•form ESC- more collisions
•activation energy lowered
•active site changes shape- products repelled from active site- different shape
Why DNA replicates before dividing?
•To ensure the full DNA component is in each daughter cell