MMB04 Bacterial Growth And Control Of Microbial Growth Flashcards

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1
Q

Where are microorganisms usually found on the body

A

On the skin and on mucous surfaces.

They should not be found on or in external organs, blood, lymph and nervous system

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2
Q

Are microorganisms usually pathogenic or benign

A

Most, but not all pathogens are benign

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3
Q

When are mammals exposed to microorganisms

A

Since mamas develop in a sterile environment, they are only exposed to microorganisms after birth from skin contact, feeding and being exposed to the environment

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4
Q

What is colonisation

A

When microorganisms begin to grow after gaining access to host tissues

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5
Q

Why are microorganisms important in gut health

A

They synthesise important vitamins like B12 and K. Some studies link a healthy gut micro biome to weight loss and mental health

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6
Q

How do microorganisms cause infections in mucus membranes

A

They may associate loosely to the mucus membrane
They may adhere strongly to the mucous and epithelial cells
They may invade deeper into the epithelial cells

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7
Q

What does infection mean

A

When a microorganisms is established and growing inside a host. This may not always be damaging to the host

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8
Q

What does disease mean

A

The damage caused to the host by the microorganisms

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9
Q

What is pathogenicity

A

The ability of a pathogen to inflict damage onto a host

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10
Q

What causes variation in pathogenicity

A

The identity of the pathogen
The susceptibility of the host

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11
Q

What is an opportunistic pathogen

A

A pathogen that only causes disease in the absence of the hosts normal resistance

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12
Q

What is virulence

A

The quantitive measure for a pathogenicity. It is the relative ability for the pathogen to cause disease

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13
Q

What are exotoxins

A

Toxic proteins released by the pathogen as it grows. They travel from the site of infection to distant areas

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14
Q

What are the three types of exotoxins

A

Cytolitic toxins
AB toxins
Superantigen toxins

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15
Q

What are cytolytic exotoxins

A

They degrade cytoplasmic membrane integrity possibly causing lysis

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16
Q

What are AB exotoxins

A

Made up of a B subunit and an A subunit. The B subunit binds to the cell membrane receptor which allows A to pass through the membrane and cause damage

17
Q

What are superantigen exotoxins

A

They stimulate large numbers of immune cells resulting in swelling and inflammation

18
Q

What are enterotoxins

A

Special exotoxins that that affect the small intestine

19
Q

What are endotoxins

A

Lipoploysacharaides produced by most gram negative bacteria. They are not secreted and are only released when the cells lyse

20
Q

What is LD50

A

The median lethal dose that can kill 50% of hosts infected

21
Q

What is pasteurisation

A

A process of heat sterilisation invented by Louis Pasteur.
Kills many organisms, but not all so is not a method of sterilisation.
Heat sterilisation will not kill endopsores

22
Q

What is autoclaving

A

Steam heating at 121 degrees C for 15mins at high pressure.
Kills all bacteria including endospores
Sterilises equipment

23
Q

How does UV light kill bacteria

A

Leads to DNA modifications and breaks causing cell death
Used do disinfect surfaces, air and water

24
Q

How does ionising radiation kill microorganisms

A

Generates electrons, hydroxyl radicals, hydride radicals which disrupt DNA, proteins and lipids
Used to sterilise equipment and food

25
Q

How do membrane filters work

A

Made of polymers that create pores too small for bacteria and spores to pass through
Usually used to sterilise liquid in labs