MLPA Flashcards
1
Q
applications of MLPA
A
multiplex ligation-dependent probe amplification
detection of deletions, duplications, aneuploidies
2
Q
MLPA steps
A
denaturation
probe hybridization
ligation
PCR
cap electrophoresis
analysis
3
Q
probe design of MLPA assay
A
two probes used
- no labels so not like a primer but like a primer bc has 3’ hydroxyl end
- right probe changes size bc of stuffer region = making different lengths = more than one bands seen
4
Q
fragments used for quality control
A
benchmark fragments
Q/quantity fragments
D fragments
X & Y fragments
5
Q
Q fragments
A
ensures the correct amount of sample DNA has been added
detects if ligation happened
6
Q
D fragments
A
ensures the denaturation step was complete
7
Q
X & Y fragments
A
can identify sample mix ups
