Mixing Studies

Question 1

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Principle of mixing studies/substitution studies

Answer 1

Substitution studies may be performed by mixing correction reagents (adsorbed plasma/aged serum), with the patient’s plasma and then performing PT and APTT to identify a specific factor deficiency.

Question 2

Adsorbed plasma is rich in factors …

Answer 2

V, VIII, XI, XII (5 8 11 12)

Question 3

Factors absent in adsorbed plasma.

Answer 3

X, IX, VII, II (1972)

Question 4

Aged serum is rich in factors …

Answer 4

7, 9 ,10, 11, 12

Question 5

Factors absent in aged serum

Answer 5

1, 2 , 5 , 8, 13
(Iyang klase kay 1 to 5 tas 8 bisag 13 pa siya)

Question 6

PT Reagent

Answer 6

Tissue thromboplastin

Question 7

APTT Reagent

Answer 7

partial thromboplastin with activator, calcium chloride

Question 8

In mixing studies, how many percent of sodium chloride is needed?

Answer 8

0.85%, (w/v)

Question 9

Type of control plasma for mixing studies

Answer 9

Normal control plasma

Question 10

Spx for mixing studies

Answer 10

Fresh citrated plasma

Question 11

Before performing the substitution test, what should be performed and why?

Answer 11

Test for circulating anticoagulants to rule out presence of an inhibitor

Question 12

Test for Factor I deficiency

Answer 12

Thrombin Time

Question 13

Factors belonging to the Instrinsic Pathway but not detected in mixing studies.

Answer 13

Prekallikrein & High Molecular Weight Kininogen (HMWK)

Question 14

Intrinsic Pathway factors in mixing studies

Answer 14

12, 11, 9, 8

Question 15

Factor/s belonging to the Extrinsic Pathway

Answer 15

7

Question 16

A factor belonging to the extrinsic pathway appearing only with injury

Answer 16

Factor 3

Question 17

Factors belonging to the common pathway

Answer 17

10, 5, (Prothrombin) 2, (Fibrinogen) 1

Question 18

PT is prolonged; APTT is normal
The missing factor belongs to ….

Answer 18

Extrinsic pathway

Question 19

PT is normal; APTT is prolonged
The missing factor belongs to …

Answer 19

Intrinsic Pathway

Question 20

Both PT and APTT are prolonged
The missing factor belongs to …

Answer 20

Common Pathway

Question 21

When adsorbed plasma is treated with Barium Sulfate or Aluminum Hydroxide, these factors will be lost.

Answer 21

Vitamin K Dependent Factors (1972)

Question 22

Labile coagulation factors that disappears when spx is left unprocessed after 2 hours.

Answer 22

Factors 5 and 8

Question 23

Most commonly used reagents

Answer 23

Fresh plasma
Adsorbed plasma
Aged serum

Question 24

Factors present in fresh plasma

Answer 24

All

Question 25

Factors present in aged plasma

Answer 25

All except 5 and 8

Question 26

Factors present in adsorbed plasma

Answer 26

All except 1972

Question 27

Factors present in fresh serum

Answer 27

All except 1, < 20% factor 2, 5, 8, 13

Question 28

Factors present in aged serum

Answer 28

All except 1, 2, 5, 8, 13

Question 29

Test for factor XIII

Answer 29

5M Urea Solubility Test/ Ducker’s

Question 30

The type of blood spx centrifuged at 2500 rpm.

Answer 30

unopened whole blood plasma

Question 31

Spx is centrifuged at 2500 rpm for 20 minutes to …

Answer 31

obtain platelet poor plasma

Question 32

Spx should be test within how many hours of collection?

Answer 32

2 hours

Question 33

Spx requirement for aged serum that is allowed to clot

Answer 33

nonanticoagulated whole blood

Question 34

Prior to use, aged serum should be diluted with

Answer 34

0.85% sodium chloride
(1 volume of serum: 4 volume of sodium chloride)