Ethanol Gelation Test Flashcards
Ethanol gelation test is designed to detect its presence in the plasma.
fibrin monomers
Ethanol gelation test is to utilized as …
- Aid in the diagnosis of DIC
- Distinguish DIC from primary fibrinolysis
Principle of Ethanol Gelation Test
When ethanol or NaOH is added to plasma containing soluble fibrin monomers, precipitation or gel is formed.
Specimen used for ethanol gelation test
Fresh citrated plasma
To obtain plasma, specimen must be…
centrifuged
How many drops of patient’s plasma is added to a test tube?
9
Drop/s of 0.1N NaOH (Sodium Hydroxide) added to plasma
1
Drops of 50% ethanol
3
The mixture of plasma, NaOH and ethanol are kept at what temperature and for how many minutes?
RT; 10 minutes respectively
What is added to the mixture when a precipitate or gel forms?
1 drop of 0.1N NaOH
Absence of a precipitate or gel formation
Negative (normal)
Presence of precipitate or gel formation
positive
sodium citrate and venous blood ratio to obtain plasma
9:1
What should be avoided in sample collection
formation of foam
Sample should be centrifuged immediately at
no less than 1500 rpm, at least 10 mins.