missed in blurts

Question 1

whats PI

Answer 1

the PH at which the net charge on an amino acid is zero

Question 2

Psi rotation can lead to?

Answer 2

to NH – NH collisions

Question 3

• Phi rotation can lead to?

Answer 3

O – O collision

Question 4

how many beta strands per beta sheet

Answer 4

2-10 strands

Question 5

what is the primary sequence of the myoglobin

Answer 5

around 150 amino acid length

Question 6

whats an allosteric inhibitor for myoglobin and what does it do?

Answer 6

lactate. lactate serves to decrease myoglobins affinity for oxygen. so lactate build up in the muscles promotes O2 release from myoglobin.

Question 7

what bonds hold the globins of Hb together

Answer 7

NON covalent

Question 8

what enzyme fixes the methaemoglobin

Answer 8

cytochrome b5 reductase

Question 9

what are the 3 weak bond types between the enzyme and substrate

Answer 9

van der waals, ionic and h bond

Question 10

what do ground state destabilisation and Transition state stabilisation have to help make T state

Answer 10

they both are achieved by having a shape that is conducive the transition state formation

Question 11

what does lipitor do and how?

Answer 11

lipitor is a cholesterol lowering drug. This acts as a transition state, reducing the abilities if HMG-CoA reductas

Question 12

irreversible inhibitors bind to the enzyme site via

Answer 12

they bind to specific amino acid side chains in the active site

Question 13

what effect does the non competitive inhibitor have

Answer 13

it binds to the enzyme changing the shape of the active site such that S still binds but transition state is no longer as stabilised