Minimal questions Mycology

Question 1

What are the fungal cell components (3 examples)?

Answer 1

-Cytoplasmic membrane with ergosterin, cell wall build up by chitin, glucan or cellulose.

Question 2

Classification of fungi by morphology?

Answer 2

Unicellular or yeast, multicellular or mould and dimorphic

Question 3

Characterisation of the dimorphic fungus:

Answer 3

They grow like molds at room temperature and grow like yeast at body temperature

Question 4

List at least two asexual spore types:

Answer 4

Blastospore, conidia, arthrospore, spherule.

Question 5

What is the cultivation temperature of the fungi?

Answer 5

25 degrees C and 37 degrees C to detect dimorphism.

Question 6

What kind of culture media can be used to cultivate fungi?

Answer 6

Sabouraud culture media.

Question 7

What are the content (2 examples) of Sabouraud culture media?

Answer 7

Antifungal agents against environmental moulds, carbohydrates, antibiotics.

Question 8

What kind of diseases can be caused by fungi?

Answer 8

Allergic reactions, intoxications, mycosis (tissue damage of fungi).

Question 9

Classification of the mycosis?

Answer 9

Superficial, cutaneous, subcutaneous, systemic, and opportunistic mycosis.

Question 10

What kind of staining can be used in mycology? (2 examples)

Answer 10

Gram staining
Methylene blue staining
India ink staining
PAS staining 
Gömöri-Grocott staining

Question 11

Mode of action of the antifungal drugs?

Answer 11

Inhibition of ergosterin synthesis,
pore formation on membrane,
inhibition of nucleic acid synthesis,
inhibition of cell division.

Question 12

List three causative agents of systemic mycosis.

Answer 12

Coccidiodes immitis
Histoplasma capsulatum
Blastomyces dermatitidis
Paracoccidiodes immitis

Question 13

List two Candida species:

Answer 13

Candida albicans
C. tropicalis
C. krusei
C. glabrata
C. parapsilosis

Question 14

List three causative agents of opportunistic mycosis:

Answer 14

Candida sp., 
Cryptococcus neoformans,
Pneumocystis jirovecii,
Aspergillus sp., 
Penicillium sp., 
Mucor sp.,
Rhizopus sp.

Question 15

Which fungus can cause subcutaneous mycosis?

Answer 15

Sporothrix shenkii

Question 16

What is the most common causative agent of superficial mycosis?

Answer 16

Malassezia furfur

Question 17

List two causative agents of dermatomycosis:

Answer 17

Trichopyton sp.,
Microsporum sp.,
Epidermophyton sp.

Question 18

What kind of diseases can be caused by dermatophytes (3 examples)?

Answer 18

Tinea pedis,
T. manus,
T. capitis,
T. corporis,
T. faciei,
T. barbae

Question 19

List two Trichophyton species:

Answer 19

Trichophyton rubrum,
T. schonleinii,
T. tonsurans,
T. verrucosum

Question 20

What are the source of the infection caused by dermatophytes?

Answer 20

Anthropophilic - human,
Geophilic - soil,
Zoophilic - animal