MIDTERMS: L3 - HEMOCYTOMETER

Question 1

MOTF

1. Hemocytometer - used to count blood cells - leukocytes, platelets, RBC
2. Count cell from body fluids such as WBCs from parietal fluid, ascitic fluid, synovial fluid, pleural fluid, pericardial fluid, and CSF
3. Cannot count sperm cells

Answer 1

1,2 - true
3 - false

3 - can count sperm cell

Question 2

Most common hemocytometer

Answer 2

Neubauer Hemocytometer

Question 3

Hemocytometer is composed of: (enumerate 2)

Answer 3

1. Heavy glass slide (meet specifications of National Institute for Standards and Technology
2. Cover glass

Question 4

MOTF
Hemocytometer
1. Each raised surface contains a ruled area used for
counting cells and is marked by precise lines etched into
the glass area
2. The raised platform surfaces are
called moats
3. The coverglass is positioned so that it covers both the
ruled areas of the hemocytometer
4. The cover glass will confine the fluid inside the chamber and regulate the depth of the
fluid

Answer 4

1 3 4 - true
2 - false

2 - depressed area called moats

Question 5

Depth of the Neubauer type is ______

Answer 5

0.1mm

Question 6

4 corner squares in the ruled area (WBC squares) are composed of _____ small squares

Answer 6

16

Question 7

Each small square in the WBC corner squares is measured ____

Answer 7

0.25mm

Question 8

T OR F

To count RBCs, use the center square, specifically the 4 corners and 1 small central square

Answer 8

TRUE (5 in total)

Question 9

T OR F

In the central large square (for RBC), the 5 squares within it is composed of 16 smaller squares

Answer 9

TRUE

Question 10

To count platelets, use the:
A. Corner squares in the large center square
B. Center square in the large center square (for RBC)
C. Center square in the 4 corner squares of WBC

Answer 10

B.

Only 1 square (the center of the large center square)

Question 11

Allow fluid to flow from the pipette and fill the entire side via ______

Answer 11

Capillary action

Question 12

T OR F

The more fluid you will charge, the more likely it will lead to bubble formation

Answer 12

False - the less fluid

Question 13

MOTF

1. Initially placed the objective in HPO in viewing hemocytometer
2. It’s easily viewed when the condenser or the light source is lowered.
3. Count in a serpentine manner

Answer 13

1 false
2,3 true

1 - LPO

Question 14

T OR F

Remember, when you count in the hemocytometer, the oil immersion objective (OIO) is NEVER used.

Answer 14

TRUE

Question 15

MOTF

1. Boundary of triple line - third line
2. Boundary of double line - second line (outer line)

Answer 15

1 false
2 true

1 - second line

Question 16

Count the cells:

1. All cells within the square
2. Cells touches left boundary
3. Cells touches right boundary
4. Cells touches top boundary

Answer 16

1,2,4

Question 17

Care of hemocytometer
CHOOSE ALL THAT APPLIES:
1. Held by side or bottom to avoid getting fingerprints
2. Do not touch ruled areas
3. Raised surfaces wiped with lens paper dipped in 10% bleach
4. Raised surfaces wiped with lens paper dipped in 70% OH
5. After use, disinfect with 10% bleach
6. After use, disinfect with 70% OH
7. If not in use, store in a container to keep dirt and dust off the surface

Answer 17

1, 2, 4, 5, 7