MIDTERMS: L1 - BLOOD SMEAR

Question 1

MOTF
Plasma - coagulated, no fibrinogen
Serum - anticoagulated; contains fibrinogen

Answer 1

BOTH F
Plasma - anticoagulated; contains fibrinogen

Serum - coagulated; devoid of fibrinogen

Question 2

Blood smear evaluate cell’s:

Answer 2

Numbers
Size
Maturity
Morphology
Structure

Question 3

WBC neutrophils with big granules (abnormal structure)

Answer 3

Toxic granulation

Question 4

Blood smear - gold standard of examining ______

Answer 4

Blood parasite - malarial infection

Question 5

Best source of specimen for blood smear

Answer 5

Capillary blood

Question 6

Capillary blood advantages and disadvantages:

Answer 6

AD: Peripheral collection and reflective of condition of the patient

DIS: Platelet clumping, limited amount

Question 7

Disadvantage of EDTA blood for blood smear:

Answer 7

Blood artifacts (if smear is stored in RT for 5 hrs)
EX:
- RBC (spherocytes, necrobiotic leukocytes and vacuolated neutrophils)
- Platelets satellitosis: Pseudothrombocyt openia

Question 8

T OR F

Blood samples collected in EDTA are stable within 12 hours of collection

Answer 8

False - stable within 4 hours

Question 9

Glass slide dimension in Manual Wedge technique

Answer 9

3in x 1in

or 75 x 25mm (rodaks)

Question 10

Features of well-made peripheral blood film:

Answer 10

1. The film is 2⁄3 to the 3⁄4 the length of the slide
2. Blood should not reach the other end of the slide
3. The film is finger-shaped
4. The lateral edges of the film is visible

5.The film is smooth without irregularities, holes,
or streaks

5.When the slide is held up to the light, to thin
portion of the film has a “rainbow” appearance

Question 11

MOTF:
1. The appropriate angle for blood smear preparation is side
20-30 degrees
2. High angle smear could be long smear

Answer 11

BOTH F
1 - 30-40 degrees
2. High angle = short, thick

Question 12

T OR F
acceptable amount of space form the edge
of the slide to the head of the smear from 0.5-1 inch

Answer 12

True

Question 13

Factors affecting thickness:

Answer 13

Thickness

• Too large drop of blood
• Too fast
• Too high angle of spreader

Question 14

Factors affecting thinness:

Answer 14

• Too thin drop of blood
• Too slow
• Too low angle of the spreader

Question 15

The pressure while pushing the slide must be at the _____

Answer 15

center - to have equal pressure

Question 16

Avoid blowing on slides to prevent moisture accumulation which lead to ________

Answer 16

echinocytic (crenated) red blood cells.

Question 17

• Rare method for peripheral Blood films

- Used for bone marrow aspirates smears

Answer 17

Coverslip Technique or Ehrlich

Question 18

Dimension of cover slips used in Coverslip Technique or Ehrlich

Answer 18

2 cover slips, 22x22mm

Question 19

Advantages of Coverslip Technique or Ehrlich

Answer 19

Excellent leukocyte distribution and Good wbc appearance

Question 20

Automated slide making and staining System

Answer 20

Beckman Coulter

Question 21

Purpose of staining smears:

Answer 21

1. Make the cells more visible and allow their
   morphology to be evaluated
2. Differentiate cells