Midterms Flashcards
routinely used for detection and size analysis of DNA and RNA
gel electrophoresis
positive electrode color
red
negative electrode color
black
other term for negative electrode
cathode
other term for positive electrode
anode
seperates fragments of dna by size
agarose gel electrophoresis
set of dna fragments that are exact multiples of 1000 bp is called
kilobase ladder
who is farther to move? linearized or supercoiled?
supercoiled
as the gel concentration increases, the movement of particles _______
slows
presence of ethidium bromide causes a _____________________
decrease in the negative charge of double stranded dna
agarose gels shoul be run at no more than ________________
5-8 V/cm
agarose is a polysaccharide polymer extracted from ___________
seaweed
most frequently used buffer
tris-acetate edta buffer
ph of tris-acetate edta buffer
ph 8.0
has very high buffering capacity
tris-borate edta buffer
ph of tris-borate edta buffer
ph 7.5.7.8
advantage of tbe
gels can be dissolved
most common for staining dna
ethidium bromide
placed in the liquid aragose after it has been poured
comb
they are used for terminating enzymic reactions
gel loading buffers
solution of dna moleculues of different length
dna ladder
most frequently applied when it is necessary to isolate large fragments of dna
electoelution
uses low melting temp
low melting point agarose
advantage of low melting point agarose
pemits recovery of large dna fragments
uses enzymes to digest agarose
agarose digesting enzyme
now the most frequently used method for recovering dna fragments
silica powder
very small dna fragments
polycacrylamide gel electrophoresis
widest application of capillary electrophoresis
capillary electrophoresis
can seperate dna molecules up to 10mb
pulsed field gel electrophoresi
