(MIDTERM) STAINING METHOD Flashcards
mainly used to detect TB bacilli, an organism that connor be stained by Gram Stain due to thick Mycolic Acid cell.
Acid Fast staining
is the preferred method because of the application of heat which makes the cell wall permeable to the primary stain, Carbol Fuochis
Ziehl Neelsen
Give 1 function of SPUTUM AFB
- Detection of Mycobaterium infection
- Diagnose tuberculosis
- Monitor the effectiveness of the treatment
- Monitor the effectiveness of the treatment
TWO TYPES OF AFB STAINING
- Kinyoun Stain Method
- Zeihl Neeisen Ston Method
also known as “Cala Method Staining”
Kinyoun Stain Method
requires no actual stringent/warming
AFB stain: Red
NOT AFB stain Green
Kinyoun Stain Method
also known as “Heat Method Staining”
Ziehl-Neelsen stain Method
uses phenol that will help to break or to dissolve the Lipias and also uses heat to penetrate the ston
Zeil Neeisen Stain Method
most commonly used method
Zeil Neeisen Stain Method
ZIEHL NEELSEN STAIN 3 components
- CARBOL FUCHSIN
- DECOLORIZER
- METHELYNE BLUE
The Primary Stain
CARBOL FUCHSIN
Counter stain in ZIEHL NEELSEN STAIN
METHELYNE BLUE
KINYOUN STAIN 4 components
- CARBOL FUCHSIN
- DECOLORIZER
- METHELYNE BLUE
- BRILLIANT GREEN
Cover whole slide with acid alcohol or ethanol and leave it until no more color drains from the smear
Decolorization
Report as Positive for AFB if present (color:)
Red
