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BIOL 200 > Midterm Proteins > Flashcards

Midterm Proteins Flashcards

1
Q

E3

A

Ubiquitin ligase

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2
Q

Hsp90

A

molecular chaperone, respond to cell stress, less common

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3
Q

RAG1/RAG2

A

used in assembly of immunoglobulin gene, domesticated transposones

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4
Q

BiP

A

ER resident molecular chaperone

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5
Q

KU80/KU70

A

heterodimer that binds ends of dsDNA in order to make them blunt, part of NHEJ

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6
Q

UPR

A

happens when too many proteins are not folded properly

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7
Q

SV40

A

virus studied in DNA polymerization

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8
Q

TriC

A

eukaryotic chaperonin protein

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9
Q

RPA

A

single-strand binding protein

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10
Q

MSH2/MSH6

A

recognize mismatched DNA in newly synthesized strands

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11
Q

SAR

A

region of DNA which associates with scaffold (non-plant)

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12
Q

Tublin

A

most common microskeleton protein

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13
Q

dsDNA

A

double stranded DNA

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14
Q

E1

A

Ubiquitin activating enzyme

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15
Q

MCM

A

eukaryote helicase

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16
Q

LINE

A

long interspersed nuclear elements

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17
Q

PCNA

A

prevents complex from disassociating from the strand

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18
Q

Trypsin

A

protease used before mass spectrometry

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19
Q

GST

A

popular affinity combination, used with gluthathione agarose

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20
Q

RFC

A

loads DNA polymerase on strand

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21
Q

MLH1 and PMS2

A

dimer, part of MMR, recognizes and cuts new strand

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22
Q

DNA glycosylase

A

strips base off of nucleotide sugar

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23
Q

MAR

A

plant region of DNA which associates with scaffold

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24
Q

ddNTP

A

dideoxyribonucleoside triphosphate

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25
Q

BER

A

base excision repair

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26
Q

BOLD

A

database used for DNA barcoding

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27
Q

PrP

A

protein that can form plaque in non-conformer state

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28
Q

DNA-PK

A

DNA dependent protein kinase, part of NHEJ

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29
Q

GTPase

A

uses GTP as on switch

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30
Q

BRCA-1/BRCA-2

A

genes on which mutations are often linked to cancer

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31
Q

Primase

A

forms RNA primer

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32
Q

DnaJ

A

works in concert with Hsp70, also known as Hsp40

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33
Q

IDP

A

type of protein domain typical of triggers

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34
Q

RecA

A

inverts strand during HR in bacteria

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35
Q

Topoisomerase

A

relieves torsional stress in dsDNA

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36
Q

APE1

A

cuts phosphodiester bond off abasic site

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37
Q

mtDNA

A

mitochondrial DNA

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38
Q

TSD

A

transposon repeats on the target site

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39
Q

FEN 1

A

helps ribonuclease H

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40
Q

Helicase

A

denatures dsDNA at replication fork

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41
Q

H3

A

histone protein which has a specialized form involved with centromeres

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42
Q

GAP

A

turns GTPase off

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43
Q

UTR

A

regions of eukaryotic primary transcript between tail and cap which aren’t translated

44
Q

HR

A

homologous recombination

45
Q

Alu

A

most common SINE in humans

46
Q

SSR

A

two types: minisatellite DNA and microsatellite DNA

47
Q

PrPsc

A

prion protein in non-conformer state

48
Q

Ribonuclease H

A

removes ribonucleotides from RNA-DNA hybrids

49
Q

Ire1

A

bound to BiP in normal circumstances

50
Q

SINE

A

short interspersed nuclear elements

51
Q

GFP

A

glows green in the dark

52
Q

MMR

A

mismatch repair

53
Q

Pol delta

A

completes Okazaki fragments, part of gap repair

54
Q

ORF1

A

RNA binding proteins, helps transport transcript back into nucleus

55
Q

E2

A

Ubiquitin conjugating enzyme

56
Q

Ira

A

GTP-ase accelerating protein that regulates RAS

57
Q

Hac1

A

translation factor in UPR

58
Q

SDS

A

used to bind proteins before gel electrophoresis

59
Q

NF1

A

mutations on this gene cause elephant-man syndrom

60
Q

Calmodulin

A

protein that binds calcium

61
Q

GEF

A

turns GTPase on

62
Q

CEN

A

sequence of DNA associated with centromeres

63
Q

IEF

A

isoelectric focusing

64
Q

CDK

A

protein that needs cyclins

65
Q

PCR

A

process for amplifying DNA

66
Q

Sma1

A

common restriction enzyme producing blunt ends

67
Q

TIR

A

inverted tandem repeats

68
Q

LTR

A

long terminal repeats

69
Q

ORF

A

area of DNA which is translated

70
Q

BLASTN

A

local alignment lookup for nucleotides

71
Q

Large-T Antigen

A

SV40 helicase

72
Q

GroEL/GroES

A

prokaryotic chaperonin protein

73
Q

Pol alpha

A

elongates DNA primer

74
Q

PDI

A

forms disulfide bridges

75
Q

XP-G / XP-F

A

endonucleases part of NER, cuts strand containing TT dimer

76
Q

IgG

A

common type of antibody

77
Q

EcoR1

A

common e-coli restriction enzyme producing sticky ends

78
Q

miRNA

A

RNA used for regulating gene expression

79
Q

Ephrin

A

ligand protein on cell membrane

80
Q

Ero1

A

resets PDI

81
Q

Taq

A

heat-resistant DNA polymerase

82
Q

DNA exonuclease

A

digests ends of DNA

83
Q

23B

A

part of NER, detects mutations

84
Q

AP lyase

A

part of Pol beta

85
Q

CO1

A

highly conserved mitochondrial gene used for DNA barcoding

86
Q

Cyclin

A

associated with a particular cell phase

87
Q

Rad51

A

inverts strand during HR in eukaryotes

88
Q

Svedburg Units

A

units of measure associated with sedimentation rate

89
Q

26S Proteasome

A

breaks down proteins into amino acids

90
Q

H1

A

histone protein which isn’t highly conserved, stabilizes 30-nm fibers

91
Q

ORF2

A

reverse transcriptase and DNA endonuclease, used for inserting LINE

92
Q

NGS

A

modern DNA sequencing

93
Q

T4 DNA Ligase

A

industry standard protein for joining DNA or RNA strands

94
Q

IR

A

sequence of DNA followed downstream by its reverse compliment

95
Q

NER

A

nucleotide excision repair

96
Q

Pol epsilon

A

leading strand extension

97
Q

BLASTP

A

local alignment lookup for proteins

98
Q

Telomerase

A

ribonucleoprotein extends lagging strand of DNA

99
Q

TFIIH

A

binds to TT dimerized strand, along with RPA, as part of NER

100
Q

CRM

A

cis regulated module

101
Q

ORI

A

origin of replication

102
Q

NHEJ

A

non-homologous end joining, fixes double strand breaks

103
Q

H2A H2B

A

histone proteins that have both C and N terminal tails

104
Q

Pol beta

A

removes abasic sites and attaches complimentary nucleotide

105
Q

ORC

A

set of proteins binding to the origin of replication

106
Q

Hsp70

A

molecular chaperone, respond to cell stress, most common

BIOL 200 (3 decks)

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