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BIOL 200 > Midterm Proteins > Flashcards

Midterm Proteins Flashcards

1
Q

E3

A

Ubiquitin ligase

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2
Q

Hsp90

A

molecular chaperone, respond to cell stress, less common

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3
Q

RAG1/RAG2

A

used in assembly of immunoglobulin gene, domesticated transposones

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4
Q

BiP

A

ER resident molecular chaperone

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5
Q

KU80/KU70

A

heterodimer that binds ends of dsDNA in order to make them blunt, part of NHEJ

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6
Q

UPR

A

happens when too many proteins are not folded properly

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7
Q

SV40

A

virus studied in DNA polymerization

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8
Q

TriC

A

eukaryotic chaperonin protein

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9
Q

RPA

A

single-strand binding protein

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10
Q

MSH2/MSH6

A

recognize mismatched DNA in newly synthesized strands

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11
Q

SAR

A

region of DNA which associates with scaffold (non-plant)

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12
Q

Tublin

A

most common microskeleton protein

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13
Q

dsDNA

A

double stranded DNA

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14
Q

E1

A

Ubiquitin activating enzyme

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15
Q

MCM

A

eukaryote helicase

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16
Q

LINE

A

long interspersed nuclear elements

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17
Q

PCNA

A

prevents complex from disassociating from the strand

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18
Q

Trypsin

A

protease used before mass spectrometry

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19
Q

GST

A

popular affinity combination, used with gluthathione agarose

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20
Q

RFC

A

loads DNA polymerase on strand

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21
Q

MLH1 and PMS2

A

dimer, part of MMR, recognizes and cuts new strand

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22
Q

DNA glycosylase

A

strips base off of nucleotide sugar

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23
Q

MAR

A

plant region of DNA which associates with scaffold

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24
Q

ddNTP

A

dideoxyribonucleoside triphosphate

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25
BER
base excision repair
26
BOLD
database used for DNA barcoding
27
PrP
protein that can form plaque in non-conformer state
28
DNA-PK
DNA dependent protein kinase, part of NHEJ
29
GTPase
uses GTP as on switch
30
BRCA-1/BRCA-2
genes on which mutations are often linked to cancer
31
Primase
forms RNA primer
32
DnaJ
works in concert with Hsp70, also known as Hsp40
33
IDP
type of protein domain typical of triggers
34
RecA
inverts strand during HR in bacteria
35
Topoisomerase
relieves torsional stress in dsDNA
36
APE1
cuts phosphodiester bond off abasic site
37
mtDNA
mitochondrial DNA
38
TSD
transposon repeats on the target site
39
FEN 1
helps ribonuclease H
40
Helicase
denatures dsDNA at replication fork
41
H3
histone protein which has a specialized form involved with centromeres
42
GAP
turns GTPase off
43
UTR
regions of eukaryotic primary transcript between tail and cap which aren't translated
44
HR
homologous recombination
45
Alu
most common SINE in humans
46
SSR
two types: minisatellite DNA and microsatellite DNA
47
PrPsc
prion protein in non-conformer state
48
Ribonuclease H
removes ribonucleotides from RNA-DNA hybrids
49
Ire1
bound to BiP in normal circumstances
50
SINE
short interspersed nuclear elements
51
GFP
glows green in the dark
52
MMR
mismatch repair
53
Pol delta
completes Okazaki fragments, part of gap repair
54
ORF1
RNA binding proteins, helps transport transcript back into nucleus
55
E2
Ubiquitin conjugating enzyme
56
Ira
GTP-ase accelerating protein that regulates RAS
57
Hac1
translation factor in UPR
58
SDS
used to bind proteins before gel electrophoresis
59
NF1
mutations on this gene cause elephant-man syndrom
60
Calmodulin
protein that binds calcium
61
GEF
turns GTPase on
62
CEN
sequence of DNA associated with centromeres
63
IEF
isoelectric focusing
64
CDK
protein that needs cyclins
65
PCR
process for amplifying DNA
66
Sma1
common restriction enzyme producing blunt ends
67
TIR
inverted tandem repeats
68
LTR
long terminal repeats
69
ORF
area of DNA which is translated
70
BLASTN
local alignment lookup for nucleotides
71
Large-T Antigen
SV40 helicase
72
GroEL/GroES
prokaryotic chaperonin protein
73
Pol alpha
elongates DNA primer
74
PDI
forms disulfide bridges
75
XP-G / XP-F
endonucleases part of NER, cuts strand containing TT dimer
76
IgG
common type of antibody
77
EcoR1
common e-coli restriction enzyme producing sticky ends
78
miRNA
RNA used for regulating gene expression
79
Ephrin
ligand protein on cell membrane
80
Ero1
resets PDI
81
Taq
heat-resistant DNA polymerase
82
DNA exonuclease
digests ends of DNA
83
23B
part of NER, detects mutations
84
AP lyase
part of Pol beta
85
CO1
highly conserved mitochondrial gene used for DNA barcoding
86
Cyclin
associated with a particular cell phase
87
Rad51
inverts strand during HR in eukaryotes
88
Svedburg Units
units of measure associated with sedimentation rate
89
26S Proteasome
breaks down proteins into amino acids
90
H1
histone protein which isn't highly conserved, stabilizes 30-nm fibers
91
ORF2
reverse transcriptase and DNA endonuclease, used for inserting LINE
92
NGS
modern DNA sequencing
93
T4 DNA Ligase
industry standard protein for joining DNA or RNA strands
94
IR
sequence of DNA followed downstream by its reverse compliment
95
NER
nucleotide excision repair
96
Pol epsilon
leading strand extension
97
BLASTP
local alignment lookup for proteins
98
Telomerase
ribonucleoprotein extends lagging strand of DNA
99
TFIIH
binds to TT dimerized strand, along with RPA, as part of NER
100
CRM
cis regulated module
101
ORI
origin of replication
102
NHEJ
non-homologous end joining, fixes double strand breaks
103
H2A H2B
histone proteins that have both C and N terminal tails
104
Pol beta
removes abasic sites and attaches complimentary nucleotide
105
ORC
set of proteins binding to the origin of replication
106
Hsp70
molecular chaperone, respond to cell stress, most common

BIOL 200 (3 decks)

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