Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIOL 200 > Final > Flashcards

Final Flashcards

1
Q

SIR 2,3,4

A

silent information regulator
recruited by RAP1/SIR1
binds to hypoacetylated histone tails
SIR2 is a histone deacetelyase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Leucine Zipper, Leu interval

A

every 7th residue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

HLH protein

A

Helix Loop Helix protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

TSIX

A

anti-sense version of XIST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

General Transcription Factors for Class 2

A 
TFIIA
TFIIB
TFIID
TFIIE
TFIIF
TFIIH
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

U2 snRNP

A

binds to intron branching point

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

SIR1

A

silent information regulator

cooperates with RAP1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

hnRNP

A

heterogenous nuclear ribonuclear proteins

associate with RNA to protect or mediate reactions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

RAP1

A

binds to DNA silencer sequences & telomeres

compacts chromatin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gcn5

A

Histone acetyl transferase

Forms co-activator with Gcn4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

class-1 transcription products

A

pre-rRNA
28S rRNA
18S rRNA
5.8S rRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

VP16 Domain

A

efficient viral activation domain, recruits host activator proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Gcn4

A

Binds to UAS in yeast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Sin3

A

HDAC scaffold protein
Binds Rpd3 among others
mSin3 in humans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

TF Activation domain

A

Protein domain that stimulates transcription

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

PNK

A

Polynucleotide Kinase - Transfers gamma phosphate from ATP to 5’ end of nucleic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

SWI/SNF

A

chromatin remodeling proteins in yeast

uses ATP to move nucleosomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Mediator complex

A

Responsible for:
looping DNA by binding to enhancers,
and recruiting general TFs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

TAF

A

TBP Associating Factors

Subunits of TFIID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

RFLP

A

Restriction Fragment Length Polymorphism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

CTD

A

Carboxy-Terminal Domain
Present on large RNA Pol 2 subunit
Heptapeptide repeat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

TFIIH Subunits

A

XPB (3’ to 5’ helicase)
XPD (5’ to 3’ helicase)
CDK7 + Cyclin H
TTDA (related to TrichoThioDystrophy)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

CTD S5 phosphorylation attractees

A 
capping enzyme
hnRNPs
splicing factors
polyadenylation factors
export factors
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

C2H2 Zinc Finger

A

2 Cys 2 His
monomers
3+ finger units

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Hometic Transformation

A

When homeotic protein is mutated, causing body plan change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Recognition Helix

A

Alpha-helical domain of protein which binds to major groove of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Common DNA Methylations

A

H3 K4 -> activates histone
H3 K9 -> deactivates histone
(K = lysine)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

CAP site

A

Promoter sequence upstream of lac operon

cAMP + CAP protein binds to it

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Intron sequence rules

A

5’ | GU A(branching point) (Py rich) AG | 3’

30
Q

Yeast mating type central loci

A

MAT, becomes MAT alpha or MATa

31
Q

alpha-amanitin

A
  • blocks RNA Pol II
  • impedes RNA Pol III
    (no effect on RNA Pol 1)
32
Q

HMT

A

histone methyl transferase

usually associated with repression

33
Q

Homeodomain

A

DNA Binding domain associated with homeotic TFs

34
Q

TFIIH functions

A 
DNA Helicase
CTD Kinase (S5)
35
Q

snRNP

A

small nuclear ribonuclear proteins

U1 U2 U4 U5 U6

36
Q

Yeast mating type locis

A

HML alpha and HMRa, on Chromosome 3

37
Q

Yeast mating types

A

alpha and “a”

38
Q

HDAC

A

histone deacetylase complex

39
Q

Location of TATA Box

A

Near [-37,-32] to [-31, -26]

Further upstream of yeast (-90)

40
Q

EtBr

A

Ethidium Bromide - Common nucleic acid stain

41
Q

PAX6

A

Developmental gene. In charge of eyes.

Some regulation sequence regions are highly conserved.

42
Q

CTD repeat

A

YSPTSPS

43
Q

Types of histone modifications

A 
acetylation
methylation
phosphorylation
ubiquination
(could be others..)
44
Q

EMSA

A

Electrophoretic Mobility Shift Assays

45
Q

Spliesome Cycle Steps

A 
U1 + U2
U1 + (U4, U5, U6) + U2
[shuffling]
U5 + U6 + U2
[transesterifications]
spliced exon + lariat intron
[debranching enzyme]
spliced exon + linear intron RNA
46
Q

DNA Acetylation

A

neutralizes positive charge on histones

makes DNA easier to access

47
Q

HAT

A

histone acetyl transferase

48
Q

GAL4

A

Binds to UAS in yeast when in low-glucose environment

C6 Zinc Finger

49
Q

U1 snRNP

A

binds to 5’ splice site

50
Q

TF Binding domain

A

Protein domain that binds to DNA

51
Q

5’ cap molecule

A

7’ methylguanylate

52
Q

ChiP

A

Chromatin immunoprecipitation

53
Q

hnRNP domains

A

RRM, KH domain, RGG repeats

54
Q

Lariat structure

A

Processed intron, RNA loop with 5’-2’ link to adenosine

55
Q

C4 Zinc Finger

A

4 Cys per unit
homo-heterodimers
2 finger units
ex: nuclear receptors

56
Q

Class-2 Transcription Initiation

A 
TFIID Binds to TATA Box
TFIIB -> TFIID
TFIIA stabilizes structure
RNA Pol II + TFFIIF -> TFIIB
TFIIE creates docking site
TFIIH + TFIIH kinase -> TFIIE

(D, B, A, F, E, H)

57
Q

Initiator

A

cis-acting promoter sequence sitting on start site
-2 to +4
weak consensus sequence (C|A usually)

58
Q

UAS

A

Upstream activating sequence (yeast promoter)

59
Q

TBP

A

TATA Box Binding Protein

Subunit of TFIID

60
Q

CTD phosphorylation proteins

A

TFIIH -> S5

p-TEF6 + CDK9, Cyclin-T -> S2

61
Q

Transcriptome

A

Snapshot of all transcripts in a cell at one point in time

62
Q

Ume6

A

Binds to URS in yeast

Recruits Sin3

63
Q

Activator domain

A

Domain on activator TFs which recruit HATs

64
Q

XIST

A

non-coding RNA that coats X chromosome to inactivate it

cis-acting

65
Q

class-2 transcription products

A

mRNA
snRNA (except U6)
siRNA
miRNA

66
Q

C6

A

6 Cys per unit

ex: GAL4

67
Q

Repressor domain

A

Domain on repressor TFs which recruit HDACs

Forms co-repressor complex

68
Q

class-3 transcription products

A 
tRNA
snRNA U6
5S rRNA
7S rRNA
(other small stable RNAs)
69
Q

Splicesome

A

Mediates splicing reaction

70
Q

Homebox genes

A

Code for homeotic proteins

71
Q

Rpd3

A

HDAC catalytic protein
histone deacetylase
binds to Sin3

BIOL 200 (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions