Midterm I - Website Photos (Oliver) Flashcards

Babesia canis: Merozoites
Blood smear

Cryptosporidium baileyi oocysts
On the ciliated cell surface of the trachea

Babesia canis merozoites
Blood smear, Giemsa-stained

Unsporulated Eimeria oocysts from a lamb

Eimeria acervulina macrogamonts & developing oocysts

Eimeria maxima: Sporulated oocyst
Iodine stain

Eimeria bakuensis: Unsporulated oocysts
Floatation method, methylene-blue stain

Eimeria: Unsporulated oocyst
E. robusta, methylene-blue stain

Eimeria stiedai: Oocysts
In destroyed biliary epithelium

Leishmania donovani: Promastigotes
Giemsa stain

Toxoplasma gondii: Tissue cysts
Mouse brain, HE stain

Trypanosoma equiperdum: Trypomastigotes
Giemsa stain

Encephalitozoon cuniculi: Focal encephalitis (see macrophages)
Rabbit brain, HE stain

Encephalitozoon cuniculi: Interstitial nephritis
Rabbit kidney, HE stain

Encephalitozoon cuniculi: Interstitial nephritis + CT proliferation
Rabbit kidney, HE stain

Eimeria bovis (larger) & Eimeria zuernii (smaller): Oocysts
Floatation, native

Leishmania donovani: Amastigotes in macrophages
Giemsa stain

Sarcocystis tenella: Tissue cyst
Sheep oesophagus, HE stain

Eimeria stiedai: Biliary hyperplasia
Rabbit liver, HE stain

Trypanosoma cruzi: Pseudocyst
In a section of heart muscle, HE stain

Trypanosoma equiperdum (Dourine): Dollar-spots on the horse

Giardia intestinalis: Cyst
Faecal smear, Lugol solution (iodine)

Giardia intestinalis: Trophozoite

Engorged tse tse fly
Responsible for the transmission of African Trypanosomes E.g:
- T. vivax
- T. congolensis
- T. brucei brucei

Histomonas meleagridis: Ameboids
Liver tissue, PAS stain

Kissing bug: Biological vector
Transmission of Leishmania chagasi (Chagas disease)

Leishmania: Amastigotes
Inside a macrophage

Cutaneous Leishmaniosis

Clinical signs of Nagana (African Trypanosmosis)
Transmitted by the Tsetse fly

Trichomonas infection (Hepatic form)
Pigeon head & liver

Sandfly: Biological vector
Transmission of Leishmania

Trichomonas: Trophozoites (Most likely: poultry)
Giemsa stain

Trypanosoma brucei: Trypomastigotes
Blood smear, Giemsa stain

Trypanosoma cruzi: Trypomastigote
+ a large kinetoplast, Giemsa stain

Lesions in the caeca & liver associated with Histomona meleagridis
Samples from a turkey

Lesions in the surface of the duodenum associated with Eimeria acervulina
Sample from a chicken

Eimeria brunetti: Bloody mucus in the lumen of the hindgut
Sample from a chicken

Macrogamonts with granular cytoplasm
One-nucleated oocysts surrounded by a wall in the destroyed wall of the intestinal mucosa

Eimeria maxima: Small haemorrhages & whitish spots of schizonts in the mucosa of the small intestine
Not to be confused with “salt & pepper” spots in E. necatrix infections

Eimeria necatrix: Haemorrhages in the jejunal wall

Nests of schizonts
Destroyed intestinal mucosa of chicken, HE stain

Eimeria tenella: Lesions of the caeca
Sample from a domestic chicken

Eimeria tenella: Lesions of the caeca
Sample from a domestic chicken

Eimeria truncata: Macrogamonts
Goose kidney tissue, HE stain

Eimeria: Sporulated oocyst
Stained with iodine

Three schizonts & free merozoites in a native mucosal smear

Coccidium spp.: Thick-walled, unsporulated oocysts

Eimeria stiedai: Biliary hyperplasia
Rabbit liver, HE stain

Clinical signs of acute bovine coccidiosis
Either E. zuernii or E. bovis

Clinical signs of Isosporosis of piglets (Isospora suis)

Eimeria stiedai: Unsporulated oocysts
These are often stained yellow by the bile

Eimeria bovis (large) & E. zuernii: Unsporulated oocysts

Section of a globidium
- Located in the top layer of interstitial mucosa*
- Eimera, rabbit or cattle*

Bovine coccidiosis (E. zuernii or E. bovis)
- Pinhead-sized white spots in the thickened mucosa of the intestine
- Covered with bloody mucus

Eimeria flavescens: Dilated caecum of a rabbit

Eimeria intestinalis: Thickened wall of small intestine
Sample from a rabbit

Eimeria magna: White groups of macroschizonts
Wall of the small intestine, rabbit

Eimeria piriformis: Thickened & whitish wall of large intestine
Sample from a rabbit

Eimeria stedai:
- Broadened parts of biliary ducts
- Yellowish nodules can be seen on the surface
Rabbit liver sample

Isospora: Sporulated oocyst

Isospora: Oocyst from:
- A dog
- A cat

Cryptosporidium baileyi:
- Thick-walled oocysts (red)
- Thin-walled oocysts (colourless)
Mucosal cell debris of bursa fabricus, Kinyoun stain

Neospora caninum: Thick-walled tissue cyst
Brain tissue, HE staining

Sarcocystis: Haemorrhagic diatheses on visceral serosa
Dalmeny disease (bo. only), Calf

Besnoitia besnoiti: Large cysts
Subcutaneous tissue of bo., HE stain

Sarcocystis gigantea: Cysts
Muscle layer of the oesophagus, sheep

Sarcocystis: Cyst (longitudinal section)
Skeletal muscle, HE stain

Besnoitia besnoti: Clinical symptoms
- Top row: “Anasarca” stage
- Bottom row: “Scleroderma” stage

Sarcocystis: Cyst (cross-section)
Skeletal muscle, HE staining

Sarcocystis: Cyst
Compressed skeletal muscle, native

Sarcocystis: Thin-walled oocyst
Faecal debris

Toxoplasma: Cysts
Brain tissue

Toxoplasma gondii: Tachyzoites
Giemsa stain

Toxoplasma: Cysts
Brain tissue, HE stain

Toxoplasma gondii: Oocysts (Sporulated & unsporulated)

Theileria annulata: Enlarged lymph nodes
(Bovine theileriosis)

Babesia canis: Clinical signs

Dermacentor reticulatus (brown dog tick/kennel dog tick)
The vector of Babesia canis

Hepatozoon canis:
- In a mononuclear cell
- In a polymorphonuclear cell

Encephalitozoonosis: Clinical sign
Interstitial nephritis

- Babesia bigemina (large)
- Babesia divergens
In blood cells

Theileria annulata: Schizonts
Taken from bovine lymphocytes

Theileria equi: Merozoites
Blood smear

Encephalitozoon cuniculi: Torticollis (clinical sign)
Rare occurance

Histomonas meleagridis: Amoeboids
Liver tissue, PAS stain

Giardia intestinalis: Trophozoites

Eimeria truncata: Macrogamonts
Goose kidney tissue, HE stain

Trypanosoma brucei: Trypomastigotes
Blood smear, Giemsa stain

Entamoeba histolytica: Trophozoite
Faecal smear + Saline + Lugol

Entamoeba histolytica: Cyst
Floatation method