Midterm Exam (Lectures 13-15)

Question 1

Name 5 potential sources of DNA from human?

Answer 1

1. blood (nucleated cells)
2. buccal swabs (epithelium)
3. saliva (lymphocytes
4. biopsy (specialized cells)
5. forensic sources (hair root, nails, etc.)

Question 2

What are the 2 methods of preparing ready to use DNA?

Answer 2

1. solid phase extraction

2. magnet beads technology

Question 3

What are the 3 main technologies for detection of SNPs?

Answer 3

1. direct sequencing (Sanger)
2. allele-specific hybridization (TaqMan)
3. primer extension (pyrosequencing and Sequenom)

Question 4

True or False - the presence of proteins or RNA in a DNA sample is considered contaminated?

Answer 4

True

Question 5

Put the following steps of TaqMan assay in order:

A) elongation of the primer/digestion of the probe
B) annealing of the probe
C) annealing of the primer
D) fluorescence of the probe

Answer 5

C (primer)
B (probe)
A (elongation)
D (fluorescence)

Question 6

Which technology for detection of SNPs is convenient for highly fragmented DNA?

Answer 6

pyrosequencing

Question 7

In pyrosequencing, each nucleotide added to the primer generates an ATP molecule. Which enzyme is used to detect ATP by giving off light?

Answer 7

luciferase

Question 8

True or False - Sequenom technology involves molecular mass of nucleotides?

Answer 8

True

Question 9

In two-dimensional electrophoresis proteins are separated according to what 2 criteria?

Answer 9

1. surface charge

2. molecular mass

Question 10

True or False - DNA microarray is used for analysis of multiple RNA molecules (gene expression) and of genetic variations (SNP Chip)

Answer 10

True

Question 11

True or False - DMET (drug metabolizing enzymes and transporters) genetic testing is not FDA approved and therefore cannot be used to test patients yet?

Answer 11

True