Midterm 2

Question 1

Anode reaction

Answer 1

H2O-> 2H +1/2 O2+ 2e

Question 2

Cathode reaction

Answer 2

2e + 2H2O -> 2 OH + H2

Question 3

Two pieces of equipment that are necessary for electrophoresis

Answer 3

1. DC power supply

2. Buffer reservoir system

Question 4

Cathode

Answer 4

gas H2

Question 5

Anode

Answer 5

gas O2

Question 6

Low voltage

Answer 6

• Paper

- Thin layer electrophoresis

Question 7

High voltage

Answer 7

• Starch
• Agarose
• PAGE
• Capillary electrophoresis

Question 8

What is agarose used for

Answer 8

to separate DNA, RNA, (small proteins)

- Nucleic acids by size

Question 9

Agarose w/v

Answer 9

0.1% - 6%

Question 10

at 0.1% with agarose

Answer 10

pore size increases, less molecules are formed when solidified

Question 11

at 6% with agarose

Answer 11

pore size decreases

Question 12

Agarose consists of

Answer 12

agarose and agaropectin

Question 13

AGE anode and cathode

Answer 13

Anode: positive O2
Cathode: Negative H2

Question 14

Pulse- Field Agarose Gel Electrophoresis (PFAGE) is used when?

Answer 14

When DNA molecule is larger than the pore size of the agarose gel
- Migrate “end-on” through the matrix as if through a sinuous tube

Question 15

Mode of migration with PFAGE

Answer 15

reptation

Question 16

Composition of PAGE

Answer 16

Acrylamide, Bis-acrylamide, ammonium persulfate, TEMED

Question 17

Acrylamide

Answer 17

polymerizes with the help of (NH4)2SO4

Question 18

Bis-acrylamide

Answer 18

cross linker

Question 19

Ammonium persulfate

Answer 19

radical donor

Question 20

TEMED

Answer 20

catalyst

Question 21

Types of PAGE

Answer 21

• Native
• Discontinuous
• SDS
• Isoelectric focusing
• 2- dimensional