Midterm 2 Flashcards
characteristics of eukaryotic cells
- presence of a nucleus
- dynamic cytoskeleton and membranes
endosymbiotic hypothesis (theory of endosymbiosis)
chloroplasts and mitochondria of eukaryotic cells were originally free-living bacteria that were incorporated into a host cell
- each have 2nd membrane and own DNA
- genes lost by transfer to nucleus
Eukaryotic kingdoms
animals, plants, fungi, protists
now 7 super kingdoms:
1) opsithokonts
2) amoebozoans
3) archaeplastids
4) stramenopila
5) alveolata
6) rhizaria
7) excavata
cytoskeleton
internal protein scaffolding – filaments of actin and microtubules
- can be remodeled quickly, enabling cells to change shape (not possible with cell wall)
- functional flexibility: movement to obtain food, vacuoles and organelles to store and digest
- enables endo/exocytosis
endo/exocytosis
membrane-lined vesicles transport through the cytoskeleton by molecular motors (much faster than diffusion) allows eukaryotes to grow in size
endomembrane system consists of:
nuclear envelope, ER, Golgi apparatus, cell/plasma membrane
phagocytosis
engulfing of food particles, even other cells
eukaryotic metabolism localized in:
mitochondria and chloroplasts
Eukaryotic DNA organization
–multiple linear chromosomes & multiple replication sites allow for rapid and simultaneous replication
(singular circular prokaryotic DNA harder for fast replication)
–non-coding DNA functions in gene expression
–more complex, different cell types, growth & development
eukaryotes w/ bacteria and archaeons
eukaryotes did not outcompete bacteria and archaeons but developed novel functions
sex promotes genetic diversity via:
1) meiotic division produces genetically unique daughter cells
2) recombination
3) independent assortment
4) fertilization – egg and sperm combinations
symbiont
organism that lives in closely evolved association with another species
protist diversity
algae - photosynthetic protists
protozoa - heterotrophic protists
some live in tests – “houses” constructed of organic molecules
Opsithokonts
- most diverse eukaryotic superkingdom (75% of all species 1.8 mil + species)
- flagellated cells - single flagellum
- (animals and fungi) heterotrophic, but some have photosynthetic symbionts
- includes choanoflagellates
- includes microsporidia: parasites inside animal cells; spores in external enviro
choanoflagellates
group of mostly unicellular protists within the superkingdom opsithokonts.
characterized by ring of microvilli: fingerlike projections that form a collar around the cell’s single flagellum
– close relationship to animals
Amoebozoans
group of eukaryotes with amoeba-like cells that move and gather food by means of pseudopodia
- more than 1000 species described
- plasmodial slime molds are coenocytic: multiple nuclei within one giant cell
- cellular slime molds aggregate into a multicellular “slug” for feeding
Archaeplastids
- 2nd most conspicuous and diverse group of eukaryotes
- includes land plants
- nearly all photosynthetic; direct descendants of protist that first evolved chloroplats from endosymbiotic cyanobacteria
- includes galucocystophytes: small group of single-celled algae found in freshwater ponds and lakes w/ more features of ancestral cyanobacterial endosymbiont than any other algae
- includes red algae
- includes green algae (viridoplantae)
red algae
- 5000+ species known, mostly marine enviro and multicellular
- principle photosynthetic pigment is chlorophyll a
- coralline algae secretes CaCO3 – live by corals – resist breaking waves
- nori, agar
green algae
from viridoplantae
- diverse assortment ~10,000 marine species, some coenocytic
- have chlorophyll a and b in chloroplasts
- unique attachment for flagella
Stramenopiles
- characterized by two flagella – one smooth, one hairy
- includes brown algae (giant kelps) and protozoa (free living cells & parasites)
- include diatoms
diatoms (stramenopiles)
- skeletons/cell walls of silica
- 1/2 of all primary production in the ocean
- 1/4 of all photosynthesis on earth (more than tropical rainforests)
- 10,000+ known species
Alveolates
cortical alveoli – small vessicles packed beneath the cell surface; in some store calcium ions for use by the cell
- dinoflagellates – photosynthetic, cellulose walls, red tide, bioluminescence
- ciliates – heterotrophic protists with two nuclei in each cell and numerous short flagella (cilia)
- apicomplexans, plasmodium species causes malaria
Excavata
- free-living, parasitic or symbiotic
- - includes euglenids (have chloroplats) and giardia (causes diarrhea)
eukaryotic lifestyle
alternation between sexual and asexual
alternation between haploid and diploid phase
animals – multicellular stage is diploid (dominates life cycle), only haploid phase is gamete
plants – 2 multicellular phases, 1 haploid, 1 diploid
advantages of sexual reproduction
- new combinations of alleles
- can produce new genotypes
- provides variation w/ new allel combos
- clears deleterious mutations
evolution of complex multicellularity
~550 MYA
– came with the rising O2 levels - oxygen necessary for multicellular life
arose six times in evolution - red algae, brown algae, green algae –> land plants, animal, fungi (twice)
bulk transport
means by which molecules move through organisms at rates beyond those possible by diffusion across a concentration gradient (i.e. pumping of blood)
– more effective than diffusion (from unicellular organisms)
Multicellular organism characteristics
1) adhesion (stick together)
2) specialized structures for communication
3) tissue and organ differentiation
4) small subset of reproductive cells
5) cell or tissue loss can be lethal to organism
6) 3-dimensional – presence of interior and exterior cells
epithelia
lining of tissues
secrete ECM
animals – cadherins, integrins, transmembrane proteins provide molecular mechanism for adhesion
plants – pectin (fruit jelly)
gap junctions
facilitate targeted transport of ions and molecules between adjacent cells in animals
plasmodesmata
facilitate targeted transport of ions and molecules between adjacent cells in plants
meristem
population of actively dividing cells at the tips of stems and roots
– permanently undifferentiated
gastrulation
movement of cells during embryogenesis that transforms the blastula –> gastrula
gastrula
brings cells into direct contact for signaling for growth and development throughout the body not just “meristem” area (in animals)
simple multicellularity
1) cell adhesion
2) little communication between cells
3) little/no differentiation of specialized cells
4) most cells have all functions and reproduction
5) every cell in contact with external enviro
selective advantages of multicellularity
1) avoid protozoan predators
2) better maintain position on a surface or in water
requirements for multicellularity
1) cell adhesion
2) communication (via gap junctions/plasmodesmata)
3) genetic program for development (network of genetic interactions for cell division and differentiation)
regulatory genes
genes that prompt expression/repression of another gene
– played important role in evolution of complex multicelluar organims
dessication
excessive water loss
vascular plants
have the ability to draw water from the soil and limit water loss from leaves
vascular plants
> 95% of all land plants today
1) ferns + horsetails
2) gymnosperms (seeded) - pine trees, conifers
3) angiosperms (seeded) - flowering plants: trees, grasses, sunflowers
4) lycophytes - forms sister group to all other vascular plants
dessication tolerance
suite of biochemical traits that allows cells to survive extreme dehydration without damage to membranes or macromolecules
- feature of bryophytes - resume photosynthesis w/ return of water
- vascular plants just draw from soil
shoot
composed of leaves, stem, and reproductive organs
roots
4th organ system
root apical meristem covered by root cap
leaf
main site of photsynthesis
- epidermis - upper and lower surface lining
- mesophyll - “middle leaf” photosynthetic cells
- veins - connects leaf to rest of the plant
CO2 and H20 exchange
CO2 diffuse in –> H20 diffuse out
hot day – water conc in mesophyll higher inside – several hundred H20 lost per CO2 for photsynthesis
transpiration
evaporative loss of water vapor from leaves
cuticle
waxy product secreted by epidermal cells to limit water loss – brought to surface by water loss (stimulation)
stomata
small pores in epidermis that regulate diffusion of gases between leaf and atmosphere
solute + H20 uptake –> opening stomata via guard cell swell
release solute + H20 –> closing stomata
fern vs. angiosperm stomataq
fern: guard cells grow and shrink independent of other epidermal cells
angiosperm: guard cell growth –> adjacent cell shrink via k+, cl- transfer, and vice versa
- increases opening, volume of guard cell up to 3x
stomatal stimulation
open: light
close: high CO2 in cell, dehydration, abscisic acid: hormone during drought–prevention
Roles of Plants
- produce oxygen
- contribute to soil (via decomposers)
- protect against erosion - hold soil together
- retain moisture in soil
- buffer local climate
human use of plants
1) agriculture - artificial selection
2) fuel sources - wood, coal
3) forestry - paper, construction
4) bio-prospecting - medicinal uses (taxol cancer drug, aspirin)
5) aesthetically pleasing
epiphytes
plants that grow high in canopy of other plants without soil contact
photoautotrophs
produce own carbon energy source (food) using sunlight
fix carbon
CO2 + H20 –> C6H12O6 + O2
parenchyma
thin walled cells enclosed by epidermis - photosynthesis
make up the mesophyll
phloem
transports carbohydrates from leaves to rest of plant
xylem
- transports water + nutrients from roots to leaves
- have pits, tracheids, and vessels
pit
circular/ovoid region in wall where water enters/exits xylem conduits
tracheid + vessel
tracheids more common in lycoophytes, ferns + horsetails, gymnosperms
- unicellular conduit, pills in secondary cell walls (transport)
vessels found in gymnosperms
- multicellular conduit (ends have gaps through 1st and 2nd wall (transport)
- individual = vessel element
evolutionary increase in vascular tissue
1) simple water-conducting cells
2) first vascular tissue (lignin)
3) tracheids (1st wall with cellulose and 2nd wall with lignin), have pills
4) vessel elements, ends have gaps through both walls
Root structures
Epidermis
- cortex: composed of parenchyma cells
- endodermis: surround xylem and phloem, controls most of nutrients into xylem
Casparian Strip
- thin band of hydrophobic material
- allows selective control of substance flow
- transport via ATP powered proteins
lots of respiration for ATP
mycorrhizae
symbioses between roots and fungi that enhance nutrient uptake
fungi “super root hairs” absorb phosphorous (phosphate ions) give phosphorous, receive carbhohydrates from roots from leaves, release enzymes that enhance PO3 motility
- consume 4-20-% of carbohydrate production
- > 85% of plant species have mycorrhizae
ectomycorrhizae
thick sheath of fungal cells surrounding root tip
endomycorrhizae
arbuscules protrude into root increases contact area
- higher C and nutrient exchange
root nodule
nitrogen fixing bacteria and root cells created when bacteria enter the root
–common in legumes, replenish nitrogen – crop rotation
phloem structures
sieve tubes, companion cell, phloem sap
sieve elements
individual cells that make up sieve tube
-linked by sieve plates - end walls w/ large pores
companion cell
carries out cellular functions (protein synthesis, etc) connected to sieve elements by plasmodesmata
phloem sap
sugar-rich solution that flows through sieve tube lumen and sieve plate pores
rhizosphere
the soil layer that surrounds actively growing roots
-rich in microbial populations that aid in decomposition
sieve tubes transport
sugars, ions, nitrogen, hormones, protein signals, RNA, moves raw material and signaling molecules
trends in plant evolution
- increase in vascular tissue
- gametophyte to sporophyte dominant life cycles (same with dependency)
- evolution of pollen: H20 independent fertilization
- evolution of the seed
bryophytes & life cycle
- mosses, liverworts, hornworts are small, simple, and tough
- many live on branches of trees or rocks (epiphytes)
- 2n zygote, 2n gametophyte, 1n spores, 1 n gametophytes, 1n gametes
- gametophyte photosynthesizes (g-phyte dependent)
- H20 dependent fertilization
- disperse via 1n spores
sporangium
structure that contains thousands of haploid spores
sporopollenin
complex mix of polymers resistant to environmental stress: UV & desiccation, that protects the zygote through the air
alternation of generations
gametophyte and sporophyte can live on their own
- one near ground for fertilization
- one above for dispersal
pollen
multicellular gametophyte within spore wall
liberated seed plants from need for swimming sperm
– allows for air dispersal