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Cc Lab > MIDTERM > Flashcards

MIDTERM Flashcards

1
Q

STATISCAL TOOLS

A

Mean
Median
Mode
Range
Standard deviation
Variance
Coefficient of variation

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2
Q

determine the concentration of an unknown sample

A

Reference calibration curve

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3
Q

calculate the limit of detection, and the limit of quantitation.

A

Reference calibration curve

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4
Q

Measure of the center of Distribution

A

Mean

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5
Q

the sum of the observed values, from the first through the last

A

Sum of X

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6
Q

Total number of observations

A

N

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7
Q

values are arranged in increasing order

A

Median

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8
Q

the value of the measured variables below which half the observation fall
- arithmetic mean of the 2 middle observation
- ex. 3,4,5,5,5,6,7

A

Median

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9
Q

value that occurs most frequently in a list of items of data.

not affected by extreme values or outliers.
- ex: 3,4,5,5,5,6,7

A

Mode

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10
Q

difference between the highest and lowest data points.
- ex: 7,6,5,5,5,4,3

A

Range

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11
Q

measure of the dispersion of values around the mean

A

Standard deviation

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12
Q

most frequently used measure of dispersion
- denoted by the use of s2

A

Variance

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13
Q

defined as a sum of the square of the deviation of the observation from the mean divided by the
total number of observations less 1

A

Variance

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14
Q

expressed as percentage of the mean

A

Coefficient of variation

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15
Q

applied to activities directed toward the monitoring of the individual elements of care.

A

Quality control

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16
Q

specimens analyzed for QC purposes.

A

Quality control materials

17
Q
  • must be available in sufficient quantity to last at least a year and aliquoted in stable form.
A

Quality control material

18
Q
  • lyophilized for stability, reconstituted in specific diluents
A

Qc materials

19
Q

solution that contains a known amount of an analyte

A

STANDARDS
- Primary
- Secondary

20
Q

Substance with an assigned value that the manufacturer establishes by using a reference method or by using reference materials traceable to primary stds.

A

Calibrator

21
Q

highly purified chemicals that can be measured directly to produce a substance of exact known
concentration.

A

Primary standard

22
Q

used for standardization of solutions of unknown strength

A

Primary standard

23
Q

stable and can be dried, preferably at 104 - 110 ̊C, without a change in composition;
- not hygroscopic

A

Primary standard

24
Q

lower purity with concentration determined by comparison with a primary standard

A

Secondary standard

25
Q

certified by the National Bureau of Standards (NBS)
- used as primary standard materials in the clinical laboratory.
- NIST and IRMM

A

Standard reference materials

26
Q

chemically and physically similar to the unknown
- specimen and is tested in exactly the same manner.

A

Control

27
Q

Monitors PRECISION of the test system
- Results: mean & SD

A

Control

28
Q

from either human serum or bovine

A

Commercial control sera

29
Q
  • Collection of excess non-hemolyzed sera without gross lipemia
A

Pooled sera

30
Q

Steps in pooled sera

A

Centrifuge -> filter -> Analyze at least 20x

Divide into 5 ml each -> stopper and store at -20 ̊C thaw

31
Q

Disadvantages of pooled sera

A

deterioration, contamination, loss of potency and pathogenic

32
Q

Ability of the method to detect even the smallest amount/slight differences in concentration.

A

Sensitivity

33
Q

Ability of the method to determine solely the compound it is supposed to measure

A

Specificity

34
Q

Indicates how close a measurement is to the true value

A

Accuracy

35
Q

Indicates how close test measurements are to each other when the same test is run on the same sample under identical conditions, time after time.

A

Precision

36
Q

Ability of a method to maintain its accuracy and precision over an extended period of time

A

Reliability

37
Q

Degree to which the method is easily repeated

A

Practicability

38
Q

CONTROL: Ideal Characteristic

A
  • Resembles human sample
  • Inexpensive and stable for long periods
  • Does NOT contain communicable diseases
  • No matrix effects
  • With known analyte concentrations
  • Convenient packaging for easy dispensing and storage

Cc Lab (2 decks)

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