Midterm 1

Question 1

Can biotin deficiency cause a build-up of ketone bodies?

Answer 1

If biotin is deficient acetyl-CoA carboxylase activity will decrease and impair the ability for fatty acids to be synthesized.

Question 2

Can biotin deficiency impair single or multiple carboxylase reactions

Answer 2

Yes

Question 3

Enzymes which use riboflavin are called

Answer 3

flavoproteins

Question 4

Deficiency in what vitamin would cause burning feet syndrome

a. niacin
b. pantothenic acid
c. thiamin
d. biotin

Answer 4

Answer: pantothenic acid

Question 5

What vitamin will be used as a coenzyme (in what form) to act as a regulator for fatty acid synthesis?

Answer 5

pantothenic acid as ACP, as fatty acid synthesis is triggered when ACP binds to fatty acid synthase (via ACP synthase) and is inhibited when ACP is removed (ACP hydrolase)

Question 6

What is the action site for biotin?

Answer 6

The action site for biotin is N

Question 7

What vitamin can be synthesized via colonic bacteria?

Answer 7

Biotin

Question 8

To separate biotin from lysine the enzyme ______ is required.

Answer 8

biotinidase

Question 9

What are the enzymes that biotin will act as a coenzyme to?

Answer 9

carboxylase enzymes

Question 10

The reaction biotin causes is

a. oxidative decarboxylation reaction
b. acyl transfer reaction
c. CO2 fixation reaction
d. redox and oxidation reactions

Answer 10

CO2 fixation reactions

Question 11

what are the four enzymes which require biotin as a coenzyme?

Answer 11

3-methylcrotonyl CoA carboxylase (leucine degradation)
pryuvate carboxylase (Energy production)
acetyl-CoA carboxylase (fatty acid synthesis)
propinyl-CoA carboxylase (TCA energy production)