Mid Year Review Part 2 Flashcards
What monomers are needed to build a protein?
Amino Acids
What part distinguishes one amino acid from another amino acid?
The R-group distinguishes one amino acid from another amino acid.
Define Primary (protein structure) and give examples
Primary – linear sequence of amino acids for a particular protein (like the sequence of letters that make up a particular word
What characteristic is crucial for the proper functioning of a protein?
Shape
What does “denaturing” a protein mean?
Shape has been disrupted by some means (usually excessive heat)
What is the name of the bond that holds two amino acids together?
Peptide
Most enzymes are what type of macromolecule?
Proteins
What term describes when an enzyme’s (or any protein’s) shape is disrupted?
Denaturation
What is the purpose of enzymes?
To catalyze (speed up) chemical reactions by lowering the barrier of activation energy
What is the name of the region on the enzyme that binds to the substrate?
Active site
What does it mean that enzymes have an optimal temperature and an optimal pH?
Optimal temp and optimal pH refer to the range of temperatures and pH a particular enzyme will work best in. Outside the range the enzyme will decrease in terms of how it can perform. (Slow down or denature)
How are competitive and noncompetive inhibition the same? How are they different?
They are similar in that the decrease an enzyme’s activity by interfering with the binding of substrate to the active site.
They are different because a competitive inhibitor will plug up an active site and block substrate from getting in while a noncompetitive inhibitor will bind to a site other than the active site. But by binding to that other site, the shape of the active site will change and prevent substrate from binding.
Are enzymes used up in a chemical reaction?
No; they can be used over and over again to speed up specific chemical reactions.
Do enzymes normally participate as a reactant or product in a chemical reaction?
Neither
Like all proteins, an enzyme’s function is highly dependent upon its _____________________.
Shape
Enzymes speed up chemical reaction by lowering _________________.
Activation energy
Why would a substance resist spontaneous breakdown?
Barrier of activation energy is too high
I want to know whether a particular inhibitor acts by competition or noncompetition. If I add more substrate, I will only affect ______________________ inhibition.
competitive
What does it mean if an enzyme is saturated?
All active sites of the enzyme are occupied (“saturated”) with substrate
If an enzyme is saturated and I want a faster yield of product, what can I do?
Add more enzyme (because this will increase the number of available of active sites).
What are the phases of the cell cycle?
Interphase (G1, S/DNA synthesis, G2) and Mitotic phase (Mitosis and Cytokinesis)
In which phase of the cell cycle does the DNA content of the cell double?
S phase
What are the four stages of mitosis?
Prophase, Metaphase, Anaphase, Telophase
Identify the major events of Prophase
centrioles move apart; formation of mitotic spindle; chromatin starting to condense
Identify the major events of Prometaphase
mitotic spindle fully formed; sister chromatids are visible; nuclear membrane is broken down and nucleolus has disappeared
Identify the major events of Metaphase
sister chromatids line up at equator of cell (metaphase plate)