Mid Year exams Flashcards
State the names of the four phases of mitosis
Prophase, metaphase, anaphase, telophase
Draw typical eukaryotic cells as they would appear during the interphase and the four phases of mitosis.
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Outline four events that occur during prophase
- DNA condenses into chromosomes and become visible
- Centrioles move to opposite poles
- The nuclear membrane starts to dissolve
- Chromosomes are comprised of genetically identical sister chromatids
Outline the process of metaphase, inclusive of the role of microtubules and the kinetochore.
- all the chromosomes line up in the middle of the cell
- spindle microtubules attach to the chromosomes at its centromere
- the attachment site is called the kinetochore
Outline the process of anaphase
- uses ATP
- sister chromatids are pulled to opposite poles
- chromatids are now considered an individual chromosome
Outline four events that occur during telophase
- chromosomes begin to uncoil
- spindle fibres disappear
- nuclear membrane reforms
- cytokinesis occurs
Describe the structure of a replicated chromosome, include the centromere and sister chromatids.
- centrioles organise spindle microtubules
- centromere is the part of a chromosome that links sister chromatids
- spindle microtubules pull chromosomes to opposite poles
- sister chromatids are duplicated chromosomes attached by a centromere
- in animal cells, two centrioles are help by a protein mass referred to as a centrosome
- after the sister chromatids separate they should then be referred to as chromosomes
Explain why chromosomes must be condensed during mitosis
Chromosomes need to be condensed to be able to fit within the nuclei of the cells
Describe the formation of the cleavage furrow in animal cell cytokinesis
- a ring of contractile protein at the equator pulls the plasma membrane inward
- the inward pull on the plasma membrane produces the characteristic cleavage furrow
- when the cleavage furrow reaches the centre of the cells it is pinched apart to form two daughter cells
Describe the formation of the middle lamella and cell wall in plant cytokinesis
- during telophase, membrane-enclosed vesicles derived from Golgi apparatus line up along the equator of the parent cell
- the vesicles fuse together to form the cell plate
- vesicles deposit, by exocytosis, pectins and other substances in the lumen between the daughter cells to form the middle lamella (gluing the cells together) of the new cell wall
- each of the two daughter cells then secrete cellulose between their new cell membrane and the middle lamella, to form the primary cell wall
State the formula for calculation of a mitotic index
Cells in mitosis divided by the total number of cells
State the location of the light independent reactions of photosynthesis
Occurs in the stroma
State that the light independent reactions of photosynthesis include:
- Carbon fixation
- carboxylation of RuBP
- Production of trios-phosphate
- ATP and NADPH as energy sources
- ATP is used to regenerate RuBP
- ATP is used to produce carbohydrates
The process of light independent reactions
- Carbon fixation: carbon dioxide enters plants and goes into stroma. The carbon dioxide attaches to ribulose bisphosphate in a process called carbon fixation (RuBP is carboxylated with carbon dioxide, catalyzed by rubisco). An unstable 6C is formed and immediately splits into 2 glycerate 3-phosphate.
- The G3P molecules are reduced by ATP and NADPH to form 2 triose phosphate molecules
- Regeneration: RuBP reforms at the expense of ATP. Most of the TP molecules produced are used to regenerate RuBP. Some of the TP are linked to form glucose phosphate
- (not a step) ATP and NADPH produced in light dependent are used in light independent
Define carbon fixation
The incorporation of inorganic carbon into organic compounds
Define carboxylation
The combination of carbon dioxide with an organic compound
State that carbon fixation occurs in the chloroplast stroma
State that carbon fixation occurs in the chloroplast stroma
State that the 5-carbon molecule ribulose bisphosphate (RuBP) is carboxylated by CO2, forming 2 3-carbon molecules called Phosphoglycerate (PGA).
• State that the 5-carbon molecule ribulose bisphosphate (RuBP) is carboxylated by CO2, forming 2 3-carbon molecules called Phosphoglycerate (PGA).
State that the enzyme that catalyzes the carboxylation of RuBP is called ribulose bisphosphate carboxylase (rubisco).
• State that the enzyme that catalyzes the carboxylation of RuBP is called ribulose bisphosphate carboxylase (rubisco).
Define variation
Individuals vary from one another in many characteristics. Variation is inherited