Microtomy Flashcards
Process of trimming and cutting the tissue block to form uniformly think slices of tissue
Microtomy
Most commonly used in microtomy
Paraffin embedded tissues
Can cut tissue sections at a pre determined thickness with the use of a knife
Microtome
Standard tissue thickness of tissue section
4-6 micrometers
It holds the block in place
Block holder
Used for cutting serial sections of large blocks of paraffin embedded tissues
Rocking microtome
Most used microtome
Rotary microtome
Cutting celloidin embedded sections
Sliding microtome
Most dangerous microtome
Sliding microtome
Used for cutting unembedded frozen sections
Freezing microtome
Used for intraoperative procedures
Freezing microtome
Used for cutting sections for electron microscopy
Ultra thin microtome
Other term for rocking microtome
Cambridge microtome
Invented rocking microtome
Paldwell Trefall
Known as the simplest microtome
Rocking microtome
When using, it will produce a slightly curved plane usually in 10-12 micrometers
Rocking microtome
Other name for rotary microtome
Minot microtome
Who invented minot microtome
Minot
What is the motion in rotary microtome
Reciprocal motion
Thickness of the block is automatically regulated by
Ratchet feed wheel and the pawl
Who invented the sliding microtome
Adams
When to use sliding microtome
Celloidein-embedded sections
Used to cut large refractory paraffin blocks
Sliding microtome
It is a type of sliding microtome suited for sectioning specimens that are embedded in all forms of media
Base-sledge microtome
Who invented the freezing microtome
Queckett
It is a bench-top type of microtome in which it uses carbon dioxide to freeze the sample
Freezing microtome
It is a refrigerated apparatus and inside is a microtome
Cryostat
Temperature maintained in a cryostat
-5 to -30 degrees celsius
The cryostat freezes the tissues within how many minutes
2-3
It is utilized for rapid preparations for urgent biopsies
Cryostat/freezing microtome
How thick is the tissue section in ultrathin microtomy
0.5 micrometers
Knife used in ultra-thin microscopy
Diamond knife
The specimen used in ultra-thin microtome is fixed in
Osmium tetroxide
Recommended side for cutting celloidin-embedded tissue blocks
Less concave side
Used to cut paraffin embedded section
More concave side
It is a 25 mm in length ; one side is flat and the other side is concave
Plane-concave knife
Both sides are concave and is recommended for cutting paraffin embedded sections in rotary microtome
Biconcave knife
Length of a biconcave knife
120 mm
Length of a plane wedge knife
100 mm
A knife that’s both sides are straight and recommended for frozen sections
Plane-wedge knife
Knife Used for cutting extremely hard and tough specimens
Plane-wedge knife
The perfect and optimum cutting angle
15 degrees
To remove the irregularities of the knife
Honing
Removal of gross nicks on the edge of the knife to remove blemishes
Coarse honing
Grinding the cutting edge of the knife on a stone to acquire an even edge angle
Honing proper
It is a natural sharping stone grinding surface
Hone
Most commonly used hone
Belgium yellow
Hone that gives more polishing effect
Arkansas
Hone that is much coarser and is used for badly nicked knives
Fine carborundum
Makes use of a vibrating frosted glass plate/wheel driven by an electrical motor
Mechanical microtome knife sharpener
Process whereby the burr formed during the honing is removed
Stropping
Polish and sharpening the cutting edge of the knife
Stropping
Best quality horse leather, firmly attached to a solid back to prevent sagging
Paddle strop
How many stroke are required in stropping
40-120 strokes
The cutting edge of disposable knives
2-4 micrometers thick
Knife used for cutting or trimming and/for semi thin sectioning of tissue blocks for electron microscopy
Glass knives
Mostly and commonly used in ultra thin microtome
Diamond knives
Cut any type of resin blocks
Diamond knives
It is brittle and expensive
Diamond knife
Preferred thickness of clean slides
1.0 - 1.2 mm
