Clearing Flashcards
Other term for de-alcoholization
Clearing
Process wherein dehydrating agent us removed from the tissue
Clearing
Clearing agent must be miscible with?
Paraffin wax
It is the measure of the bending of the ray of light that passes through one medium to another medium
High index of refraction
Aside from removing alcohol, a clearing agent must also be miscible with
Canada balsam
Other term for xylene
Xylol
It is the best clearing agent
Xylene
It clears within 15-30 mins
Xylene
It is the most rapid clearing agent
Xylene
Substitute for xylene and benzene
Toluene
It takes 1-2 hrs for this clearing agent to finish
Toluene
It takes 15-60 minutes to clear
Benzene
This is recommended for urgent biopsies and for routine purposes
Benzene
Preferred by some as a clearing agent in the embedding process
Benzene
This is not a true clearing agent
Chloroform
Used for clearing tissue in the embedding process even up to those tissues in 1cm in thickness
Chloroform
This is recommended for tough tissues
Clearing
This is used to clear both paraffin and celloidin sections during the embedding process
Cedar wood oil
This should be filtered before use
Cedarwood oil
This is recommended for clearing embryos, insects and very delicate specimens
Aniline oil
This clearing agent is not recommended due to its tendency to become adultered
Clove oil
These are slow-acting clearing agents that can be used when double embedding techniques are required.
Methyl Benzoate and Methyl Salicylate
