MICROSCOPY [TYPES OF MICROSCOPES]

Question 1

This microscope uses visible light that passes through the speciment via series of lenses.

Answer 1

Light Microscope

Question 2

This uses a beam of electron and elecromagnetic fields instead of lenses.

Answer 2

Electron Microscope

Question 3

This uses web-based interfaced and usually automated type of microscope.

Answer 3

Digital Microscope

Question 4

These are the 4 types of light microscopes.

Answer 4

• Brightfield
• Darkfield
• Phase-contrast
• Fluorescence

Question 5

These are the 2 types of electron microscope.

Answer 5

• Transmission Electron Microscope
• Scanning Electron Microscope

Question 6

During the 1950’s, they constructed the first simple compound microscope.

Answer 6

FH. & Z. Janssen

Question 7

In 1665, they developed the first laboratory compound microscope.

Answer 7

Robert Hooke

Question 8

They developed the modern classroom microscope.

Answer 8

Kepler and Galileo

Question 9

In 1672, they developed the first simple microscope with a magnification of 200x to 300x.

Answer 9

Leeuwenhoek

Question 10

This is the most used light microscope, posesses a bright background, and usually stained specimens.

Answer 10

Brightfield Microscope

Question 11

This makes use of a “stop”, inserted in the condenser, blocking light that will enter the objective.

Has a dark backrgound, and the specimen is illuminated.

Answer 11

Darkfield Microscope

Question 12

This is used to examine internal structures and motility of living organisms.

Does not need staining.

Answer 12

Phase-contrast Microscope

Question 13

This is developed by Haitinger and Coons.

Mercury Vapor Arc Lamp as the light source.

Has a dark background and light specimens.

Answer 13

Fluoresence Microscope

Question 14

This is the substance specimens are stained in orde to be fluorescent.

Answer 14

Fluorochromes

Question 15

Some substances innately posses fluorescent properties and is visualized when UV llight is used.

Answer 15

Auto-fluorescence

Question 16

These are substances that has auto fluorescent qualites.

Answer 16

• Chlorophyll
• Riboflavin
• Vitamin A
• Collagen

Question 17

These are substances that are usually stained by fluorochromes in order to be visualized.

Answer 17

• Cellulose
• Starch
• Glycogen
• Proteins
• Y Chromosome

Question 18

What are the uses of the following fluorochromes and the color visualized:

• Acridine Orange
• Diamidino-2-Phenylindole (DAP)
• Fluorescein Isothiocyanate (FITC)
• Tetramethylrhodamine Isothyiocyanate (TRITC or Rhodamine)

Answer 18

• Acridine Orange (DNA) (Orange)
• DAP (DNA) (Green)
• FITC (Antibodies) (Green)
• TRITC or Rhodamine (Antibodies) (Red)

Question 19

Who, in 1932, developed the electron microscope?

Answer 19

Knoll and Ruska

Question 20

This electron microscope is used to view detailed internal structures of cells.

Has great resolution and specimens must be sectioned into thin slices and covered with stained or metal coats.

Has a resolving power of 0.2nm.

150,000x to 10,000,000x magnification.

Answer 20

Transmission Electron Microscope

Question 21

This electron microscope is used for studying the topography of solid objects.

Used a beam of electrons at low energy in order to create a 3D image.

Has a resolving power of 200mm.

20x to 10,000x Magnification

Answer 21

Scanning Electron Microscope