MICROSCOPY [INTRODUCTION]

Question 1

Used in order to magnify small objects cannot be seen by the naked eye.

Answer 1

Microscope

Question 2

This is the ratio of an object’s size seen under the microscope to its actual size.

Answer 2

Magnification

Question 3

This is the ability to distinguish between two very samll objects.

Answer 3

Resolution

Question 4

What are the objective lens, their magnification, and total magnification.

Answer 4

• Scanning (4x) (40x)
• Low (10x) (100x)
• High (40x) (400x)
• Oil Immersion (100x) (1000x)

Question 5

This is the ability to differentiate two cloe points as separate within a minimum distance.

The eye can differentiate 0.25mm

Light microscopes can differentiate 0.25um apart.

Electron microscopes can differentiate 0.5nm apart.

Answer 5

Resolving Power

Question 6

This is the distance between the objective lens and the slide.

This generally decreases as magnification increases.

Answer 6

Working DIstance

Question 7

This is the ratio of the lens diameter to its focal length.

Serves as an index of resolving power.

Directly proportional to the resolution of the image.

Answer 7

Numerical Aperture

Question 8

What is the corresponding NA with each objective lens?

Answer 8

• 4x (0.1 NA)
• 10x (0.25 NA)
• 40x (0.65 NA)
• 100x (1.25 NA)

Question 9

What are the parts of a microscope?

Answer 9

• Ocular Lens
• Body
• Arm
• Revolving Nosepiece
• Objective Lens
• Stage
• Stage Clips
• Condenser (Focuses Light)
• Diaphragm (Light Passes)
• Illuminator
• Corase Knob
• Fine Knob
• Base

Question 10

These are the three main types of microscopes.

Answer 10

• Light Microscope
• Electron Microscope
• Digital Microscope