Microscopy ID

Question 1

Answer 1

Nisseria Meningitidis

Question 2

Answer 2

Staphylococci

Question 3

Answer 3

Actinomyces

Question 4

Answer 4

Campylobacter

Question 5

Answer 5

E. Coli

Question 6

Answer 6

Nocardia

Question 7

Answer 7

Streptococcus pneumoniae

Question 8

Clinical Utility of Gram Stain

Answer 8

1. Direct Examination of clinical material for initial diagnosis
2. Quality of Specimine
3. Bacterial Identification aid
   
   • Gram positive= retain dye complex
   • Gram negative = loses purple color after decolorization
4. Clinical Relevance of the culture

Question 9

Gram Stain Procedure

Answer 9

1. Cells on the slide
2. Primary Stain Crystal Violet
3. Mordant Grams iodine
4. decolorizer alcohol and/or acetone
5. Counterstain safranin

Question 10

Organisms stain positive for Acid Fast Stain

Answer 10

1. Mycobacteria
2. Nocardia
3. Cryptosporidia
4. Cyclospora

Stain bacteria with cell walls containing long chain FAs

Question 11

Fungal Stain Types & Examples

Answer 11

1. Lactophenol cotton blue
   
   • Histoplasma
2. Gomori Methenamine silver (GMS)
   
   • Aspergillus
   • Blastomyces dermatitidis
3. Periodic acid-Schiff (PAS)
   
   • Aspergillus
   • Blastomyces dermatitidis
4. Mucicarmine (Mucin)
   
   • Cryptococcus capsule

Question 12

Parasite Stains & Examples

Answer 12

1. Wright’s Giemsa (Certain Bugs Really Try my Patience)
   
   • Chlamydia
   • Borrelia
   • RIckettsia
   • Trypanosomes
   • Plasmodium
2. Wet Mounts
   
   • Giardia
3. Trichrome
   
   • Giardia
4. Modified Acid Fast
   
   • Nocardia