Microscopy

Question 1

What has Microscopy given the chance for people to do?

Answer 1

Microscopes have given people the chance to observe microscopic details.

Or

Magnify what cannot be seen by the human eye.

Question 2

What can you observe, using it?

Answer 2

Allows use to observe cell organelles

Question 3

What are “Organelles”?

Answer 3

Specialised structures found within a living cell.

Question 4

What is “Light Microscopy”?

Answer 4

This is the use of visible light and magnifying lenses to observe small objects.

Question 5

Name one positive of “Light Microscopy”?

Answer 5

You can observe sub-cellular structures

Question 6

Name one limitation of “Light Microscopy”?

Answer 6

Lower magnification (x500) and resolution (x200nm)

Question 7

What is “Electron Microscopy”?

Answer 7

The use of an electron filled beam in a vacuum with a wavelength of less than 1nm to visualise the specimen.

Question 8

Name one positive of “Electron Microscopy”?

Answer 8

x500000 magnification, high resolution (0.1nm) electron micrographs produced.

Question 9

Name one limitation of “Electron Microscopy”?

Answer 9

They destroy the sample

Question 10

How do you calculate Magnification?

Answer 10

Magnification = Size of Image (I) / Actual Size (A)

Question 11

How do you calculation Actual Size/Resolution?

Answer 11

Actual size/Resolution = Image size / Magnification

Question 12

A microbiologist measures an electron micrograph image of a bacterium to be 4.5cm In length. The magnification used to view the bacterium was 22500x. Calculate the actual size of the bacterium.

Answer 12

4.5cm
45mm x 1000 =
45000μm / 22500 =
2μm

Question 13

What is “Magnification”?

Answer 13

The number of times larger the image appears compared to the actual size of the object being viewed.

Question 14

What is “Resolution”?

Answer 14

The ability to distinguish between objects that are close together.