Microscopy

Question 1

Resolution

Answer 1

How well a microscope distinguishes between two points that are close together

Question 2

Light microscopes

Answer 2

Work by passing light through the specimen

Question 3

Electron microscopes

Answer 3

Use electrons rather than light.

Higher magnification and resolution - can see smaller things in much more detail

Question 4

Microscope Practical

Answer 4

• take a clean slide and use a pipette to our one drop of water in the middle of it - secure specimen in place, then use tweezers to places you specimen on the slide
• add stain if specimen is transparent - makes specimen easier to see
• place cover slip at one end of the specimen, holding it at an angle with a mounted needle and lower it onto the slide. Press it down gently so no air bubbles are trapped. Clip the slide onto the stage.
• select the lowest powered objective lens
• use coarse adjustment knob to move stage up so the slide is just underneath the objective lens, looking down at the eyepiece move the stage downwards.
• adjust the focus with the fine adjustment knob until a clear image. Position a clear ruler on the stage and use it to measure the diameter of the circular area visible

Question 5

Millimetre x 1000

Answer 5

Micrometre (x10-6m)

Question 6

Micrometre x 1000

Answer 6

Nanometre (x10-9m)

Question 7

Nanometre x 1000

Answer 7

Picometre (x10-12m)