Microscopy Flashcards
Light Microscope
Any microscope that uses light
Ocular Lens
the lens that is looked through
Objective Lens
One near the slide
Parfocal
describes the microscope staying relatively focused as magnification increases
Parcentric
slide remains centered as magnification increases
Bright Field Microscopy
the image is darker than the background. Lenses are causing convergence. The convex lens focus the light (focal point), distance between the lens and F is the focal length
Resolving Power
the minimum distance between 2 objects with them still observed as separate entities. As resolution becomes better, resolving power decreases
What factors impact resolutions path of light? (resolution dependent on)
Quality and size of lens
Oil Immersion
Wavelength
Numerical Aperture
A measure of the cone of light entering into the objective lens
Working Distance
distance between the edge of the objective lens and the surface of the specimen
Relationship between oil and glass
They have the refractive index
Dark Field Microscope
Staining not required
Dark background, bright organism
collects only the light reflected and refracted by the ORGANISM
This is used for eukaryotic cells and larger bacteria
Phase Contrast Microscope
No staining is required
Increases contrast by slowing down some wavelengths
Good for prokaryotes
Has 2 types of rings- annular: directs light around and through the specimen
phase: increase the contrast of light waves by speeding them up
Fluorescence Microscopes
UV light source
Excites fluorochromes
Fluorescent dyes
Confocal Microscopy
Laser examines 3D objects in multiple planes by a computer
Normally fluorescently stained
