Microscopy Flashcards
When were microscopes 1st developed?
mid-1600’s
Modern microscopes offer both higher ____ and higher _____.
Magnification, resolution
What is resolution?
The distance between two points that can be discriminated as distinct points.
What are the lenses of a light microscope (From bottom of microscope to top).
Condenser lens, specimen, objective lens, ocular lens, eye/viewing screen.
What is the equation for resolution (d)?
d= (0.61*lambda)/(NA) NA= (n * sin alpha)
Modern microscopes have lenses with higher _____ (light gather ability)
Numerical aperture (NA)
What is magnification?
Enlargement value
How can you manipulate the wavelength to improve resolution (lower d)
Make the wavelength shorter
Compared to the light microscope, electron microscopes have the same _______ but higher ________.
Magnification, resolution
How does the electron microscope improve resolution?
Decreases the wavelength greatly (beam of electrons).
Specimen coated with _____ get better diffraction of electrons under the electron microscope.
heavy metals
What is x-ray diffraction?
Process where x-rays are diffracted from atoms in protein crystal onto radiation sensitive plates. Higher resolution than Electron microscopy.
Epi-fluorescence microscopy uses _______ that excites _________ on the molecule?
single light wavelength, fluorescent label
What are the two main filters on the Epi-fluorescence microscope?
Excitation filter (allows light only of excitation wavelength of probe to reach specimen) and barrier filter (allows only light of emission wavelength of probe to reach eyepiece).
Know in vitro labeled, immunofluorescence and GFP tagging.
Okay.
