Microscopy Flashcards
A microscope with a high resolution and uses two sets of lenses providing a 2-dimensional image of the sample.
Compound Microscope
A magnified image of the object is obtained by the objective lens. This image is known as the __________.
real image
The eyepiece or the ocular lens then magnifies the real image more and is viewed as the ____________.
virtual image
It is a U-shaped structure and supports the entire weight of the compound microscope.
Foot or base
It is a vertical projection. This stands by resting on the base and supports the stage.
Pillar
The entire microscope is handled by a strong and curved structure known as the_________.
arm
The flat and rectangular plate that is connected to the arm’s lower end. The specimen is placed here for studying and examining the various features. The centre has a hole through which light can pass.
Stage
It is a joint, wherein the arm is fastened to the compound microscope’s pillar. The microscope can be tilted using this.
Inclination joint
The upper part of the stage is connected to these. The slide can be held in its position with their help.
Clips
It is fastened below the stage. It controls and adjusts the intensity of light that passes into the microscope.
Diaphragm
Two types of diaphragms
Disc diaphragm
Iris diaphragm
It is circular and a rotating metal part that is connected to the body tube’s lower end. This has three holes wherein the objective lenses are embedded.
Nose piece
The upper part of the arm of the microscope comprises a hollow and tubular
structure. This can be shifted down and up using the adjustment knobs.
Body tube
It is the smaller knob, which is used for sharp and fine focusing of the object. For accurate and sharp focusing, this knob can be used.
Fine adjustment knob
It is a large knob that is used for moving the body tube down and up for bringing the object to be examined under exact focus.
Coarse adjustment knob
At the top of the body tube, this lens is planted. There are certain markings such as 5X, 10X, 15X, etc. These indicate the magnification power. The object’s magnified image can be observed with the help of this.
Eyepiece lens/Ocular
It is found attached either to the pillar or the lower end of the arm. It can be used for reflection of light rays into the microscope.
Mirror
At the bottom of the body tube, there are two of these, which are connected to the revolving nosepiece.
Objective Lens
What are the four objective lenses?
a. Oil immersion objective
b. High power objective
c. Low power objective
d. Scanning objective
What is the objective lens magnification of scanning power?
4x
What is the objective lens magnification of low power objective?
10x
What is the objective lens magnification of high-power objective?
40x
What is the objective lens magnification of oil immersion objective?
100x
It magnifies the image produced by the microscope’s objective so that it can be seen by the human eye.
Eye piece
It separates the objectives and the eyepiece and assures continuous alignment of the optics.
Body tube
Holds the objective lenses and attaches them to the microscope head.
Nose Piece
Gathers light from the specimen, magnifies the image of the specimen, and projects the magnified image into the body tube.
Objective Lens
It is where the specimen to be viewed is placed.
Stage
Gather wavefronts from the microscope light source and concentrate the, into a cone of light that illuminates the specimen with uniform intensity over the entire view field.
Condenser
To reflect light from an external light source up through the bottom of the stage.
Mirror
To control the amount and shape of light passing through the optical system, thereby influencing the illumination and contrast of the specimen.
Iris Diaphragm
It serves as a support for the microscopes.
Base
Tilt your microscope back for more comfortable viewing.
Inclination Joint
Hild the slides in place.
Stage clip
Used to bring the specimen into sharp focus under low power and is used for all focusing when using high power lenses.
Fine adjustment knob
Used to bring the specimen into sharp focus under low power and is used for all focusing when using high power lenses.
Coarse Adjustment Knob
