Microscopy Flashcards
What do electron microscopes use to form an image?
Electron microscopes use electrons instead of light to form an image.
How does the magnification of electron microscopes compare to light microscopes?
Electron microscopes have a much higher magnification than light microscopes.
What is resolution in microscopy?
Resolution is the ability to distinguish between two points; a higher resolution gives a sharper image.
What is the formula for calculating magnification?
The formula for magnification is: magnification = image size / real size.
What is the first step in preparing a slide for microscopy?
Add a drop of water to the middle of a clean slide.
How do you prepare an onion cell slide?
Cut up an onion, peel off epidermal tissue, place it in water on the slide, add iodine solution, and cover with a cover slip.
What are the main parts of a light microscope?
The main parts include the eyepiece, coarse adjustment knob, fine adjustment knob, high and low power objective lenses, stage, and light.
What is the first step when using a light microscope?
Clip the prepared slide onto the stage.
How should you draw observations made under a microscope?
Draw observations neatly with a pencil, ensuring it takes up at least half the space, is drawn with straight lines, and includes labels.
What should be included in your drawing from the microscope?
Include a title of what you observed and the magnification used.
What does the coarse adjustment knob do?
Moves the stage up and down.
What do the objective lenses do?
Decreases or increase magnification.
What does the fine adjustment knob do?
Focus the image, especially when at a higher magnification level.
Why should you stain the specimen with iodine?
To highlight details by adding colour to them.
Why should there be no air bubbles?
They can obstruct the view of the specimen.
