Microscopy Flashcards
Magnification
Enlargement of the specimen
Objective lens
Magnifies the specimen
Ocular lens
Magnifies the Real Image from the objective lens
10x
Total magnification
objective lens x ocular lens
4x objective x 10x ocular len=40x total
Resolving power
Ability of a lens to show two adjacent objects as discrete entities
Determined by wavelength
Refractive index
bending power of light
Contrast
Ability to distinguish an object from its background
Depth of focus
“thickness” of a sample that increases with magnification
Field of view
Area of slide being currently observed
Working distance
Distance between the objective and the slide
be able to distinguish types of blood cells, including white blood cell