Microscopy

Question 1

Define Magnification

Answer 1

How much larger the image appears compared to the actual image size
M=IS/OS

Question 2

Define Resolution

Answer 2

The ability to distinguish two close points as separate, determining the clarity and detail of the image.

Question 3

Define Field of View

Answer 3

The visible area under the microscope

Question 4

define Artefact

Answer 4

drying out tissue using solvents like acetone

Question 5

what is fixation

Answer 5

coating the specimen in heavy metals such as lead so the electrons wont go through but bounce off creating an image. Used in Electron Microscopy

Question 6

define Staining

Answer 6

injecting in a resin (e.g. araldite) into the specimen to make it hard

Question 7

what is dehydration

Answer 7

preserving structures by making cross links in the tissue, using chemicals like glutaraldehyde and osmium tetroxide

Question 8

what is embedding with resin

Answer 8

using a light microscopy to increase the contrast and improve visibility

Question 9

what does staining with heavy metals do?

Answer 9

visible structural detail causes by processing the specimen but not a feature of the specimen (e.g. air bubbles and mesosomes)