Microscopy Flashcards
The technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye.
Microscopy
Instrument that can be used to observe small objects, even cells.
Microscope
A type of microscope that is commonly used i clinical laboratories.
Compound light microscope
Means that id one objective is in focus and a change is made to another objective the focus will bot be lost.
Parfocal
4 Parts of Microscope
- Framework
- Illumination system
- Magnification system
- Focusing system
firm, horseshoe-shaped foot on which the microscope rests.
Base
structure that supports the magnifying and adjusting systems
Arm
horizontal plate form, on which the object being observed is placed.
Stage
Manipulate the object being observed.
Mechanical stage
The type of illumination system most often employed in the clinical laboratory.
Brightfield microscope
controls the light intensity.
Rheostat
directs and focuses the beam of light form the bulb onto the material under examination.
Condenser
controls the amount of light passing through the material under observation.
Aperture Iris Diaphragm
controls the area of the circle of light in the field of when the specimen and condenser have been properly focused.
Field diaphragm
The lens that magnifies the image formed by the objective.
Ocular (Eyepiece)
Responsible for primary image formation and play a central role in determining the quality of images.
Objectives
It is used to study the unstained structures, which can be alive. Because wet preparations of cells or organisms are observed without prior dehydration and staining.
Phase-contrast microscope
Technique give the viewer a three-dimensional image of the object under study.
Interference - Contrast Microscpe
It is often used in geology for particle analysis and in forensic medicine clinically.
Polarizing Microscope
It is used in the routine clinical laboratory to observe rochetes in exudates from leptospiral or syphilitic infection.
Darkfield microscope
It is used in the clinical laboratory particularly in the clinical microbiology laboratory and for various immunologic studies.
Fluorescence Microscope
2 types of Electron Microscope
- Transmission
- Scanning
It may used for good resolution with 50,000 s magnification.
Electron Microscope
