Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIOL 321 > Microscopy > Flashcards

Microscopy Flashcards

1
Q

Who was the first person to observe microorganisms accurately?
A) Francesco Stelluti
B) Robert Hooke
C) Antony van Leeuwenhoek
D) Kathy Park Talaro

A

C) Antony van Leeuwenhoek

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the focal length of a lens related to?
A) The brightness of the image
B) The distance between the lens and the specimen
C) The distance between the center of the lens and the focal point
D) The magnification strength of the lens

A

C) The distance between the center of the lens and the focal point

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What happens when air is replaced with immersion oil in microscopy?
A) The numerical aperture and resolution decrease.
B) The lens becomes less effective.
C) More light rays enter the objective lens, increasing numerical aperture and resolution.
D) The working distance decreases.

A

C) More light rays enter the objective lens, increasing numerical aperture and resolution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Which type of microscope is best suited for observing live cells without staining?
A) Brightfield
B) Darkfield
C) Phase-Contrast
D) Fluorescence

A

C) Phase-Contrast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Which microscopy technique is most appropriate for visualizing 3D surfaces?
A) Transmission Electron Microscopy (TEM)
B) Scanning Electron Microscopy (SEM)
C) Fluorescence Microscopy
D) Brightfield Microscopy

A

B) Scanning Electron Microscopy (SEM)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is a key difference between light microscopes and electron microscopes?
A) Light microscopes use electron beams, while electron microscopes use light.
B) Electron microscopes have a higher resolution than light microscopes.
C) Light microscopes produce 3D images, while electron microscopes produce 2D images.
D) Light microscopes require specimen preparation, while electron microscopes do not.

A

B) Electron microscopes have a higher resolution than light microscopes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The Brightfield microscope produces:
A) A dark image against a bright background
B) A bright image against a dark background
C) A 3D image of the specimen’s surface
D) High-contrast images without staining

A

A) A dark image against a bright background

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Which type of microscope is used to observe internal structures in eukaryotic microorganisms?
A) Brightfield Microscope
B) Phase-Contrast Microscope
C) Dark-Field Microscope
D) Fluorescence Microscope

A

B) Dark-Field Microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Phase-Contrast microscopy enhances contrast by:
A) Using fluorescent dyes
B) Creating 3D surface images
C) Producing a bright image against a dark background
D) Converting phase shifts in light into changes in light intensity

A

D) Converting phase shifts in light into changes in light intensity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Fluorescence microscopy utilizes:
A) Electrons to illuminate specimens
B)dyes or proteins that emit light when excited
C) Light reflected off the specimen
D) Interference of light waves

A

B) Fluorescent dyes or proteins that emit light when excited

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the primary difference between Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM)?
A) TEM provides 3D images, while SEM provides 2D images.
B) TEM produces high-resolution images of internal structures, while SEM provides 3D images of surfaces.
C) SEM uses light to visualize specimens, while TEM uses electron beams.
D) TEM requires thicker specimen sections than SEM.

A

B) TEM provides high-resolution images of internal structures, while SEM provides 3D images of surfaces

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In Scanning Electron Microscopy (SEM), how is the image of the specimen created?
A) By passing electrons through the specimen
B) By using fluorescent dyes to tag specific structures
C) By scanning a narrow electron beam over the specimen and detecting secondary electrons
D) By converting phase shifts in light into high-contrast images

A

C) By scanning a narrow electron beam over the specimen and detecting secondary electrons

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Which microscopy technique requires the specimen to be under vacuum?
A) Brightfield Microscopy
B) Phase-Contrast Microscopy
C) Dark-Field Microscopy
D) Transmission Electron Microscopy (TEM)

A

D) Transmission Electron Microscopy (TEM)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What does the working distance refer to in microscopy?
A) The distance between the lens and the focal point
B) The distance between the front surface of the lens and the specimen when in sharp focus
C) The distance between the specimen and the light source
D) The distance between the ocular lenses and the objective lenses

A

B) The distance between the front surface of the lens and the specimen when in sharp focus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Which microscopy technique is particularly useful for studying the localization of specific proteins in cells?
A) Dark-Field Microscopy
B) Fluorescence Microscopy
C) Phase-Contrast Microscopy
D) Brightfield Microscopy

A

B) Fluorescence Microscopy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Which type of light microscope is ideal for observing live, unstained organisms?
A) Brightfield
B) Darkfield
C) Phase-Contrast
D) Fluorescence

A

B) Darkfield

17
Q

What is the main advantage of using immersion oil with the oil immersion objective?
A) It reduces the working distance.
B) It decreases the contrast of the image.
C) It increases the numerical aperture and resolution.
D) It allows for the use of a thicker specimen.

A

C) It increases the numerical aperture and resolution

18
Q

Which electron microscopy technique provides high-resolution images of internal structures by passing electrons through thin sections of the specimen?
A) Scanning Electron Microscopy (SEM)
B) Transmission Electron Microscopy (TEM)
C) Confocal Microscopy
D) Fluorescence Microscopy

A

B) Transmission Electron Microscopy (TEM)

19
Q

In Scanning Electron Microscopy (SEM), what type of image is produced?
A) 3D images of surfaces
B) 2D images of internal structures
C) Brightfield images
D) Fluorescent images

A

A) 3D images of surfaces

20
Q

What is a key feature of Confocal Microscopy?
A) Uses fluorescent dyes for tagging cellular structures
B) Provides detailed 3D images through laser scanning
C) Creates high-contrast images of live cells
D) Produces bright images against dark backgrounds

A

B) Provides detailed 3D images through laser scanning

21
Q

What is a primary advantage of electron microscopy over light microscopy?
A) Electron microscopy is less complex and requires less specimen preparation.
B) Electron microscopy provides higher resolution, allowing visualization of smaller structures.
C) Light microscopy can produce 3D images of specimen surfaces.
D) Light microscopy allows for more detailed study of microbial morphology.

A

B) Electron microscopy provides higher resolution, allowing visualization of smaller structures.

22
Q

Which of the following types of microscopy uses phase shifts in light to enhance contrast without staining?
A) Fluorescence Microscopy
B) Dark-Field Microscopy
C) Phase-Contrast Microscopy
D) Brightfield Microscopy

A

C) Phase-Contrast Microscopy

23
Q

The total magnification of a microscope is determined by:
A) The ocular lenses multiplied by the objective lenses
B) The lens strength alone
C) The focal length of the lenses
D) The working distance and the numerical aperture

A

A) The ocular lenses multiplied by the objective lenses

24
Q

Which microscopy technique is best for tagging specific cellular structures or molecules with fluorescent dyes?
A) Dark-Field Microscopy
B) Phase-Contrast Microscopy
C) Brightfield Microscopy
D) Fluorescence Microscopy

A

D) Fluorescence Microscopy

25
Q

What does a high numerical aperture indicate in microscopy?
A) A lower magnification
B) A larger working distance
C) Increased resolution
D) Reduced contrast

A

C) Increased resolution

26
Q

What type of image is typically produced by a Brightfield microscope?
A) High-contrast images of live cells
B) 3D surface images
C) Dark images against a bright background
D) Fluorescent images

A

C) Dark images against a bright background

BIOL 321 (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions