Microscopy Flashcards
Types of microscopy
LM: Light microscopy
EM: Electron microscopy
TEM: Transmission electron microscopy
SEM: Scanning electron microscopy
Process of staining
Fixation -> Embedding -> Sectioning -> Staining
What does fixation do in the staining process?
Stops cell breakdown
What does embedding do in the staining process?
Gives structure
What does sectioning do in the staining process?
Slice so it can be looked thru
What does staining do in the staining process?
Enhances structure
What are the types of stains?
Basophilic
Acidophilus
Trichrome
Artifact
Basophilic Stain is shown as what color?
Blue & Purple
Basophilic stains interact with what structures
Acidic structures
Acidophilic stains are what colors?
Pink, red and orange
Acidophilic stains interact with what structures?
Basic structures
Trichrome stains what?
Collagen
Trichrome stain is what color?
Blue & Green
What is artifact?
Tissue shrinkage or lost molecules
Usually white/pale color