Microscopy Flashcards

1
Q

Types of microscopy

A

LM: Light microscopy
EM: Electron microscopy
TEM: Transmission electron microscopy
SEM: Scanning electron microscopy

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2
Q

Process of staining

A

Fixation -> Embedding -> Sectioning -> Staining

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3
Q

What does fixation do in the staining process?

A

Stops cell breakdown

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4
Q

What does embedding do in the staining process?

A

Gives structure

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5
Q

What does sectioning do in the staining process?

A

Slice so it can be looked thru

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6
Q

What does staining do in the staining process?

A

Enhances structure

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7
Q

What are the types of stains?

A

Basophilic
Acidophilus
Trichrome
Artifact

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8
Q

Basophilic Stain is shown as what color?

A

Blue & Purple

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9
Q

Basophilic stains interact with what structures

A

Acidic structures

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10
Q

Acidophilic stains are what colors?

A

Pink, red and orange

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11
Q

Acidophilic stains interact with what structures?

A

Basic structures

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12
Q

Trichrome stains what?

A

Collagen

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13
Q

Trichrome stain is what color?

A

Blue & Green

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14
Q

What is artifact?

A

Tissue shrinkage or lost molecules
Usually white/pale color

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