Microscopy

Question 1

What are the objective lenses

Answer 1

scanning -4x

low-power- 10x

High power- 40x

oil-immersion-100x

Question 2

What is the magnificantion of all ocular lenses?

Answer 2

10x

Question 3

Find the total magnification of the lenses;

scanning-4x

low-power- 10x

High-power- 40x

oil-immersion- 100x

Answer 3

scanning= 40x

low-power= 100x

high-power= 400x

oil-immersion= 1000x

Question 4

Based on this formula, the shorter the wavelength,
the greater the

Answer 4

resolving power of the lense

Question 5

. Decreasing the wavelength
will not automatically increase the resolving
power of a lens, because

Answer 5

the visible portion of
the electromagnetic spectrum is very narrow and borders on the very short wavelengths found in
the ultraviolet portion of the spectrum.

Question 6

Resolving power is also dependent on

Answer 6

the refractive index

Question 7

This is
the bending power of light passing through air
from the glass slide to the objective lens

Answer 7

refractive index

Question 8

A rule of thumb is that as the magnification of the lens increases, the distance between the
objective lens and slide, called working distance,

Answer 8

decreases, whereas the numerical aperture of the objective lens increases

Question 9

working distance: The smaller the objective ex 4x

Answer 9

The further away the working distance, the bigger the magnification the smaller the working distance