2. Flashcards
How do we see bacteria?
We have to make slides
What is a bacterial smear?
- Thinly spread bacteria on a slide.
Staining is when?
we dye the bacteria so that it can be seen against a background
What is the charge of staind
Stains are usually positively charged dyes that are attracted to the negatively charged nucleic acid and cell wall
What are the common basic stains?
include methylene blue, crystal violet, and carbol fuchsin
The steps of slides
1) First, we spread thinly spread some bacteria on a slide
This is called a bacterial smear
2) Then, we dye the bacteria so that it can be seen against a background
This is called staining
Stains are usually positively charged dyes that are attracted to the negatively charged nucleic acid and cell wall
Common basic stains include methylene blue, crystal violet, and carbol fuchsin
uses one color to help us see bacteria against the background
simple staining
help to identify and differentiate different types of bacteria
dfferentrial stain
What are the 5 differentrial stains
-Gram stains are a type of differential stain
-Negative Staining
-Acid-Fast Staining
-Endospore Staining
Bacteria have a thick cell wall made of the
polysaccharide peptidoglycan
Gram-positive bacteria color and cell wall
- purple
-Thick cell wall
Gram-negative bacteria color and cell wall
Pink and thin cell wall